Description

4-Methylumbelliferyl 2-Acetamido-2-Deoxy-4,6-o-(p-Methoxyphenylmethylene)-α-D-Galactopyranoside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of specific glycosidase enzyme activity. This compound matters because it provides a high signal-to-noise ratio in enzymatic assays, enabling precise biochemical research and diagnostic development. It is primarily needed by researchers and manufacturers in the fields of glycobiology, enzymology, and diagnostic kit production for studying carbohydrate-active enzymes.

Application

• Glycosidase Enzyme Assays: A critical fluorogenic substrate for the detection and kinetic analysis of specific α-galactosidases and related enzymes.
• Biochemical Research: Used in high-throughput screening (HTS) platforms to identify enzyme inhibitors or activators for drug discovery.
• Diagnostic Development: Employed in the formulation of clinical diagnostic kits for the detection of enzymatic deficiencies, such as Fabry disease.
• Cell Biology Studies: Applied in histochemistry and cytochemistry to localize enzyme activity within tissues and cells.
• Quality Control in Biomanufacturing: Used to monitor the activity and purity of enzyme preparations in pharmaceutical production.
• Academic Research: A key reagent for universities and research institutes studying carbohydrate metabolism and lysosomal storage disorders.

Basic Information

Item	Details
Product Name	4-Methylumbelliferyl 2-Acetamido-2-Deoxy-4,6-o-(p-Methoxyphenylmethylene)-α-D-Galactopyranoside
CAS No.	1042999-77-8
Molecular Formula	C₂₇H₂₉NO₁₁
Molecular Weight	543.52 g/mol
Synonyms	4-MU-α-D-GalNAc-4,6-(p-methoxyphenylmethylene); 4-Methylumbelliferyl α-D-GalNAc-4,6-(p-methoxyphenylmethylene); Fluorogenic α-GalNAcase substrate; 4-MU-α-GalNAc-4,6-PMP; 4-Methylumbelliferyl N-acetyl-α-D-galactosaminide derivative; p-Methoxyphenylmethylene-protected 4-MU-α-GalNAc; 4-MU-α-D-GalNAc-4,6-O-(p-methoxybenzylidene)
EINECS	Contact for details

Quality Control

Every batch of our 4-Methylumbelliferyl 2-Acetamido-2-Deoxy-4,6-o-(p-Methoxyphenylmethylene)-α-D-Galactopyranoside undergoes rigorous analytical testing to ensure high purity and consistent performance in sensitive biochemical assays. Our quality protocols are designed to meet the exacting standards of research and diagnostic applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Fluorogenic Activity	Functional in standard enzyme assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.