Description

Biotin-Peg2-C4-Alkyne is a high-purity, multifunctional chemical reagent designed for advanced bioconjugation and labeling applications. This compound matters for its unique trifunctional design, combining biotin's strong affinity for streptavidin, a hydrophilic PEG2 spacer for improved solubility, and a terminal alkyne group for efficient copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry. It is essential for researchers and manufacturers in pharmaceutical development, proteomics, and diagnostic assay creation who require precise and stable labeling of biomolecules.

Application

• Bioconjugation and Protein Labeling: Site-specific modification of proteins, antibodies, and peptides for imaging, pull-down assays, and therapeutic agent development.
• Proteomics Research: Enrichment and isolation of biotinylated target proteins or complexes using streptavidin/avidin-based solid supports.
• Diagnostic Kit Development: Synthesis of detection probes for ELISA, lateral flow assays, and other immunoassay platforms.
• Chemical Biology & Drug Discovery: As a versatile building block for creating chemical probes to study protein function, interactions, and cellular pathways.
• ADC (Antibody-Drug Conjugate) Linker Exploration: Used in the design and synthesis of novel linker-payload constructs for targeted cancer therapies.
• Materials Science: Functionalization of surfaces, nanoparticles, and polymers with bioactive ligands for biosensor and biomaterial applications.

Basic Information

Product Name	Biotin-Peg2-C4-Alkyne
CAS No.	1011268-28-2
Molecular Formula	C₂₃H₃₉N₅O₅S
Molecular Weight	497.65 g/mol
Synonyms	Biotin-PEG2-C4-Alkyne; Biotin-PEG2-tetraethylene glycol-alkyne; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(14-(prop-2-yn-1-yloxy)-3,6,9,12-tetraoxatetradecyl)pentanamide; N-(Biotinoyl)-N'-(propargyl-PEG2)-C4-amide; Biotinylated Alkyne PEG2 Linker; Alkyne-PEG2-Biotin
EINECS	Contact for details

Quality Control

Our Biotin-Peg2-C4-Alkyne is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure identity, purity, and consistency. Certificates of Analysis (COA) with detailed specifications are provided for every shipment, supporting compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.