Description

Bis(2-(4-Azidosalicylamido)Ethyl) Disulf is a specialized heterobifunctional crosslinking agent containing a photoreactive aryl azide group and a disulfide bond. This compound matters for its unique ability to form covalent bonds upon UV irradiation, enabling the study of protein-protein interactions, ligand-receptor binding, and the construction of advanced biomaterials. Researchers and manufacturers in bioconjugation, proteomics, and pharmaceutical development require this reagent for photoaffinity labeling and creating cleavable, stimuli-responsive crosslinked networks.

Application

• Photoaffinity Labeling: A critical tool for mapping binding sites and identifying molecular targets of bioactive compounds in drug discovery.
• Bioconjugation & Immobilization: Used to covalently attach proteins, peptides, or other biomolecules to surfaces, nanoparticles, or polymers under controlled UV light activation.
• Proteomics Research: Facilitates the study of protein complexes and interaction networks through crosslinking mass spectrometry (XL-MS) workflows.
• Stimuli-Responsive Materials: The disulfide bond allows for reductive cleavage (e.g., by DTT or TCEP), enabling the design of smart drug delivery systems and degradable hydrogels.
• Antibody-Drug Conjugate (ADC) Development: Serves as a linker candidate for constructing cleavable ADCs, leveraging the reducible disulfide bridge for payload release in target cells.
• Surface Modification: Functionalizes gold surfaces or other materials for biosensor and diagnostic assay development.

Basic Information

Product Name	Bis(2-(4-Azidosalicylamido)Ethyl) Disulf
CAS No.	199804-21-2
Molecular Formula	C30H28N8O8S2
Molecular Weight	692.72 g/mol
Synonyms	N,N'-(Disulfanediylbis(ethane-2,1-diyl))bis(4-azido-2-hydroxybenzamide); 4-Azidosalicylamide disulfide derivative; Photo-cleavable heterobifunctional crosslinker; Aryl azide disulfide crosslinker; BS(PEG)5 analogue (photoactive); SASD (Sulfosuccinimidyl 4-azidosalicylamide homobifunctional crosslinker) related compound; Azido-SS crosslinker; Contact for details on additional trade names.
EINECS	Contact for details

Quality Control

Our Bis(2-(4-Azidosalicylamido)Ethyl) Disulf is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing to ensure high purity, identity confirmation, and functional integrity. We provide comprehensive Certificates of Analysis (COA) with data from HPLC, NMR, and mass spectrometry. Our quality commitment aligns with standards for research-grade biochemical reagents, supporting reproducible and reliable experimental outcomes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. The product is light-sensitive (store away from light) and should be handled under subdued lighting conditions. Allow the vial to equilibrate to room temperature in the dark before opening to minimize moisture condensation. For optimal performance, use immediately after preparation of solutions.

Specification

Item	Specification
Appearance	Yellow to light brown powder or crystalline solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.