Description

4-Methylumbelliferyl-β-D-Glucuronide Trihydrate is a high-purity fluorogenic substrate widely used in enzymatic assays. This compound is critical for the sensitive and specific detection of β-glucuronidase activity, a key biomarker in various diagnostic and research applications. It is an essential reagent for pharmaceutical R&D, clinical diagnostics, and microbiological laboratories requiring precise enzyme activity measurements.

Application

• Clinical Diagnostics: As a substrate for detecting β-glucuronidase activity in diagnostic kits, particularly for identifying specific bacterial strains like E. coli in water and food safety testing.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) assays to identify and characterize inhibitors or activators of β-glucuronidase for drug discovery.
• Microbiology: Employed in microbial identification and enumeration, leveraging the enzyme's presence as a marker for certain microorganisms.
• Biochemical Research: A fundamental tool for studying enzyme kinetics, mechanism of action, and substrate specificity of β-glucuronidase in vitro.
• Cell Biology & Reporter Assays: Used in cellular systems to monitor gene expression or enzyme activity when linked to a reporter gene construct.
• Quality Control in Manufacturing: Applied in the quality assurance of products where microbial contamination or specific enzymatic activity must be monitored.

Basic Information

Product Name	4-Methylumbelliferyl-β-D-Glucuronide Trihydrate
CAS No.	199329-67-4
Molecular Formula	C16H16O10 • 3H2O
Molecular Weight	398.31 g/mol (Anhydrous basis: 344.29 g/mol)
Synonyms	4-MUG; 4-Methylumbelliferyl β-D-glucuronide trihydrate; MUG; 4-Methylumbelliferyl-β-D-glucopyranosiduronic acid trihydrate; β-Glucuronidase substrate; 4-MU-β-D-Glucuronide; 7-Hydroxy-4-methylcoumarin β-D-glucuronide trihydrate; 4-Methylumbelliferyl glucuronide
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl-β-D-Glucuronide Trihydrate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data. We can supply material suitable for research, diagnostic, and pharmaceutical development applications, with specifications aligned with industry best practices.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent hydrolysis and maintain stability. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	10.0 - 14.0% (Theoretical for Trihydrate: ~13.6%)
Fluorogenic Activity	Suitable for enzyme assays
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.