Description

5'-o-Tritylthymidine 3'-Ce Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer essential for automated oligonucleotide synthesis. This compound is a critical building block for the solid-phase synthesis of DNA and RNA sequences, enabling precise control over genetic material assembly. It is primarily required by biotechnology, pharmaceutical, and diagnostic industries for applications in therapeutic oligonucleotide development, genetic research, and molecular diagnostics.

Application

• Oligonucleotide Synthesis: As a key monomer for the automated solid-phase synthesis of DNA strands, particularly for sequences containing thymidine.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides (ASOs), siRNA, and aptamers for pharmaceutical applications.
• Research & Development: Synthesis of custom primers, probes, and gene fragments for PCR, sequencing, and genetic engineering in academic and industrial labs.
• Diagnostic Probe Production: Manufacture of labeled and unlabeled DNA probes for diagnostic assays and microarray technologies.
• Scale-Up Synthesis: Suitable for process development and GMP-grade production of oligonucleotide-based active pharmaceutical ingredients (APIs).

Basic Information

Product Name	5'-o-Tritylthymidine 3'-Ce Phosphoramidite
CAS No.	198637-50-2
Molecular Formula	C43H47N4O7P
Molecular Weight	762.83 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)thymidine 3'-(2-Cyanoethyl N,N-diisopropylphosphoramidite); DMT-dT-CE Phosphoramidite; Thymidine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; dT-CE Phosphoramidite; DMT-Thymidine Phosphoramidite; 5'-DMT-Thymidine-3'-CE Phosphoramidite; T-CE Phosphoramidite; Amidite T
EINECS	Contact for details

Quality Control

Our 5'-o-Tritylthymidine 3'-Ce Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in oligonucleotide synthesis. Each lot is subjected to rigorous analytical testing, including HPLC for purity and 31P NMR for structural confirmation, to meet the stringent requirements of research and GMP environments. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and solvent residues is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This material is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms
Identification (31P NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Moisture (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.