Description

H-Glu-Ala-Leu-Phe-Gln-Pna is a synthetic peptide substrate designed for high-precision biochemical research and diagnostic applications. This compound is critical for studying and quantifying the activity of specific proteolytic enzymes, such as aminopeptidases and other proteases. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers who require reliable and sensitive tools for enzyme kinetics and inhibitor screening studies.

Application

• Enzyme Activity Assays: Used as a specific chromogenic substrate for measuring the kinetic parameters and activity levels of target proteases in research settings.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel enzyme inhibitors for drug discovery programs.
• Diagnostic Development: Serves as a key component in the formulation of clinical diagnostic kits for detecting specific enzyme-related pathologies.
• Biochemical Research: Facilitates fundamental studies on protease specificity, catalytic mechanisms, and substrate recognition.
• Quality Control in Biologics: Applied in the QC testing of therapeutic enzymes to ensure batch-to-batch consistency and potency.

Basic Information

Product Name	H-Glu-Ala-Leu-Phe-Gln-Pna
CAS No.	198551-00-7
Molecular Formula	C36H49N9O11
Molecular Weight	783.83 g/mol
Synonyms	Glutamyl-Alanyl-Leucyl-Phenylalanyl-Glutaminyl-p-Nitroanilide; EALFQ-pNA; H-Glu-Ala-Leu-Phe-Gln-4-Nitroanilide; Peptide Substrate 198551-00-7; Protease Substrate EALFQ-pNA; pNA Substrate EALFQ; L-Glutamyl-L-alanyl-L-leucyl-L-phenylalanyl-L-glutaminyl 4-nitroanilide
EINECS	Contact for details

Quality Control

Our H-Glu-Ala-Leu-Phe-Gln-Pna is manufactured under strict quality management systems to ensure the highest standards of purity and batch consistency, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assay conditions. We support compliance with relevant research and development guidelines.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Performance in Assay	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.