Description

6-Chloro-3-Indolyl α-D-Galactopyranoside is a chromogenic substrate specifically designed for the detection of α-galactosidase enzyme activity. This compound matters for its high sensitivity and specificity in biochemical assays, providing a clear, insoluble blue indigo dye upon enzymatic cleavage. It is primarily needed by research institutions, diagnostic kit manufacturers, and biotechnology companies for applications in molecular biology, enzymology, and genetic screening.

Application

• Enzyme Histochemistry & Cytochemistry: Used as a substrate for the visual localization of α-galactosidase activity in tissue sections and cell cultures.
• Molecular Biology Research: Essential in cloning and genetic engineering for blue-white screening of recombinant bacteria expressing the lacZα complementation system when used with specific vectors.
• Diagnostic Assay Development: A key component in the formulation of kits for diagnosing genetic disorders like Fabry disease, which involves α-galactosidase A deficiency.
• Microbiology: Employed in agar plates for the differentiation and identification of microbial species based on α-galactosidase production.
• Plant Biology Studies: Used to study enzyme activity and gene expression related to carbohydrate metabolism in plants.
• Quality Control in Biotechnology: Acts as a reagent for quantifying α-galactosidase activity in enzyme preparations and bioprocess monitoring.

Basic Information

Product Name	6-Chloro-3-Indolyl α-D-Galactopyranoside
CAS No.	198402-61-8
Molecular Formula	C20H24ClNO11
Molecular Weight	489.86 g/mol
Synonyms	6-Chloro-3-indolyl-α-D-galactopyranoside; X-α-Gal; 5-Bromo-4-chloro-3-indolyl-α-D-galactopyranoside (related analog); α-D-Galactopyranoside, 6-chloro-3-indolyl; Chromogenic α-galactosidase substrate; Gal-α-6Cl; 6-Cl-IGP; 6-Chloro-1H-indol-3-yl α-D-galactopyranoside
EINECS	Contact for details

Quality Control

Our 6-Chloro-3-Indolyl α-D-Galactopyranoside is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and spectroscopic identification (IR, NMR), to ensure it meets high-purity research-grade standards. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. For long-term storage, consider keeping the product at -20°C under desiccant to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Melting Point	Contact for details
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.