Description

8-Bromo-5'-O-(4,4'-Dimethoxytrityl)-N2-Dimethylaminomethylene-2'-Deoxyguanosine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite is a high-purity, protected phosphoramidite monomer essential for the automated solid-phase synthesis of oligonucleotides. This compound is critical for introducing modified guanosine bases into synthetic DNA strands, enabling advanced research and therapeutic development. It is primarily used by biotechnology and pharmaceutical companies engaged in antisense drug discovery, diagnostic probe development, and fundamental nucleic acid research.

Application

• Oligonucleotide Synthesis: Key building block for the automated, solid-phase synthesis of DNA sequences containing modified guanosine nucleotides.
• Antisense & siRNA Therapeutics: Production of research-grade and therapeutic oligonucleotides designed for gene silencing and targeted modulation of gene expression.
• Diagnostic Probe Development: Manufacture of labeled probes and primers for PCR, sequencing, and microarray applications that require specific base modifications.
• Structural Biology Studies: Incorporation of brominated nucleosides for phasing in X-ray crystallography studies of nucleic acid-protein complexes.
• Aptamer & SELEX Technology: Synthesis of chemically modified DNA libraries to enhance binding affinity and nuclease resistance for aptamer selection.
• Basic Nucleic Acid Research: Tool for studying DNA structure, function, and interactions through site-specific introduction of a heavy atom (bromine) or a protected base.

Basic Information

Product Name	8-Bromo-5'-O-(4,4'-Dimethoxytrityl)-N2-Dimethylaminomethylene-2'-Deoxyguanosine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite
CAS No.	198080-37-4
Molecular Formula	C48H55BrN7O7P
Molecular Weight	952.88 g/mol
Synonyms	8-Bromo-dG Phosphoramidite; 8-Bromo-2'-deoxyguanosine Phosphoramidite; 5'-O-DMT-8-Bromo-N2-DMF-dG Phosphoramidite; 8-Br-dG-CE Phosphoramidite; N2-Dimethylaminomethylene-8-bromo-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyguanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]phosphoramidite; 8-Bromo-5'-O-DMT-N2-DMF-2'-deoxyguanosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Guanosine, 2'-deoxy-8-bromo-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N-[(dimethylamino)methylene]-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]
EINECS	Contact for details

Quality Control

Our 8-Bromo-dG Phosphoramidite is manufactured under strict quality control protocols to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is analyzed by HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and the absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (31P NMR)	Spectrum corresponds to reference standard
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.