Description

Fluorescein Monophosphate Monoammonium is a high-purity, water-soluble substrate specifically designed for enzymatic assays. This compound is critical for detecting and quantifying the activity of alkaline phosphatase (ALP) and other phosphatases, serving as a key reagent in diagnostic and research applications. It is essential for manufacturers and researchers in the life sciences, clinical diagnostics, and pharmaceutical development sectors who require reliable, sensitive, and specific enzyme substrates.

Application

• Clinical Diagnostic Kits: Primary substrate for detecting alkaline phosphatase (ALP) activity in ELISA, immunoblotting, and automated clinical chemistry analyzers.
• Biomedical Research: Used in enzyme kinetics studies, cell-based assays, and high-throughput screening (HTS) for phosphatase activity.
• Pharmaceutical Development: Employed in drug discovery and development for screening potential enzyme inhibitors or activators.
• Molecular Biology: Acts as a chromogenic and fluorogenic substrate in nucleic acid hybridization detection systems and blotting techniques.
• Food Safety & Environmental Testing: Utilized in biosensor development and assay kits for detecting bacterial contamination or environmental pollutants.
• Quality Control (QC) in Biotechnology: Standard reagent for the quality control of diagnostic reagents and assay validation.

Basic Information

Item	Details
Product Name	Fluorescein Monophosphate Monoammonium
CAS No.	197777-68-7
Molecular Formula	C20H17NO10P
Molecular Weight	462.32 g/mol
Synonyms	Fluorescein Monophosphate Ammonium Salt; FMP Monoammonium Salt; 6-Fluorescein Monophosphate Ammonium Salt; Fluorescein-6-Phosphate Monoammonium Salt; AMP-Fluorescein; Alkaline Phosphatase Substrate FMP; 3',6'-Bis(phosphonooxy)fluoran monoammonium salt; Fluorescein 6-Phosphate
EINECS	Contact for details

Quality Control

Our Fluorescein Monophosphate Monoammonium is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and spectrophotometric analysis for performance validation. A detailed Certificate of Analysis (COA) is provided with every shipment, ensuring traceability and compliance with research and diagnostic-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is light-sensitive and should be handled under appropriate conditions to prevent degradation. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Clear, yellow solution in water or buffer
Enzymatic Activity	Suitable for use as ALP substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.