Description

Gal Beta(1-4)Glcnac Beta(1-3)Gal Beta(1-4)Glc-β-Pnp is a well-defined synthetic oligosaccharide derivative, specifically a p-nitrophenyl (PNP) glycoside of a branched tetrasaccharide. This compound serves as a critical high-purity biochemical substrate and research tool for studying glycosidase and glycosyltransferase enzyme activity, kinetics, and specificity. It is essential for researchers and manufacturers in the pharmaceutical, diagnostic, and life science research sectors who require precise tools for glycobiology studies, enzyme inhibitor screening, and carbohydrate-based assay development.

Application

• Enzyme Substrate for Glycosidases: Used as a chromogenic substrate for the assay and characterization of β-galactosidases and related enzymes, where cleavage releases the yellow p-nitrophenol for easy spectrophotometric detection.
• Glycosyltransferase Research: Acts as an acceptor molecule in studies investigating the activity and specificity of glycosyltransferases involved in complex carbohydrate synthesis.
• Inhibitor Screening & Drug Discovery: Employed in high-throughput screening (HTS) platforms to identify and evaluate potential inhibitors of carbohydrate-processing enzymes, a target area for various therapeutic applications.
• Diagnostic Reagent Manufacturing: Serves as a key raw material in the formulation of diagnostic kits and reagents for clinical enzymology and metabolic disorder testing.
• Glycobiology & Structural Studies: Provides a defined oligosaccharide structure for investigating carbohydrate-protein interactions, cell signaling, and the biological roles of glycoconjugates.
• Biochemical Standard & Reference Material: Used as a certified reference standard for quality control and method validation in analytical laboratories.

Basic Information

Product Name	Gal Beta(1-4)Glcnac Beta(1-3)Gal Beta(1-4)Glc-β-Pnp
CAS No.	197526-33-3
Molecular Formula	C32H48N2O23
Molecular Weight	828.73 g/mol
Synonyms	p-Nitrophenyl β-lacto-N-neotetraoside; PNP-β-LNnT; pNP-LNnT; 4-Nitrophenyl O-β-D-galactopyranosyl-(1→4)-2-(acetylamino)-2-deoxy-β-D-glucopyranosyl-(1→3)-O-β-D-galactopyranosyl-(1→4)-β-D-glucopyranoside; Lacto-N-neotetraose p-nitrophenyl glycoside; LNnT-PNP; Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ-PNP
EINECS	Contact for details

Quality Control

Our high-purity biochemical grade Gal Beta(1-4)Glcnac Beta(1-3)Gal Beta(1-4)Glc-β-Pnp is manufactured under controlled conditions. Each batch undergoes rigorous analytical testing including HPLC, NMR, and MS to confirm identity, purity, and structural integrity. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.