Description

4-Methylumbelliferylbeta-D-Ribofuranoside is a specialized fluorogenic substrate used for the enzymatic detection of glycosidase activity. This compound is critical for biochemical research and diagnostic applications, where it serves as a highly sensitive probe to measure specific enzyme kinetics and activity levels. It is primarily utilized by researchers and manufacturers in the fields of biochemistry, molecular biology, and diagnostic assay development.

Application

• Enzymatic Substrate for Glycosidases: Acts as a fluorogenic substrate for β-D-ribofuranosidase and related enzymes, releasing fluorescent 4-methylumbelliferone upon cleavage.
• Biochemical Research Reagent: Used in enzyme kinetics studies, inhibition assays, and characterization of glycosidase function in metabolic pathways.
• Diagnostic Assay Development: Employed in the design of fluorometric assays for clinical diagnostics and high-throughput screening (HTS) platforms.
• Microbiology and Cell Biology: Applied in studies of bacterial and fungal glycosidases, as well as in cellular activity profiling.
• Pharmaceutical Screening: Utilized in drug discovery programs to identify and evaluate potential glycosidase inhibitors.
• Quality Control in Enzyme Production: Serves as a standard reagent for the quality assessment and activity calibration of commercial enzyme preparations.

Basic Information

Product Name	4-Methylumbelliferylbeta-D-Ribofuranoside
CAS No.	195385-93-4
Molecular Formula	C15H16O8
Molecular Weight	324.28 g/mol
Synonyms	4-Methylumbelliferyl β-D-Ribofuranoside; 4-MU-β-D-Ribofuranoside; 4-Methylumbelliferyl-β-D-riboside; 7-Hydroxy-4-methylcoumarin β-D-ribofuranoside; β-D-Ribofuranoside, 4-methylumbelliferyl; 4-MU-Rib; Fluorogenic ribofuranoside substrate
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferylbeta-D-Ribofuranoside is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectral identification (UV, IR), and moisture content analysis. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term stability, store desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Solubility	Soluble in DMSO, Methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.