Description

4-Trifluoromethylumbelliferyl Tetra-o-Acetylated α-D-n-Acetylneuraminate Methyl Ester is a specialized fluorogenic substrate and synthetic intermediate used in advanced biochemical research and development. This compound is critical for the study and detection of neuraminidase (sialidase) enzyme activity, offering high sensitivity and specificity. It is primarily utilized by researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors for enzyme assay development, inhibitor screening, and glycobiology studies.

Application

• Fluorogenic Substrate for Neuraminidase/Sialidase: A key reagent for the continuous, highly sensitive fluorometric assay of neuraminidase (sialidase) enzyme activity in research and diagnostic applications.
• Enzyme Inhibitor Screening: Used in high-throughput screening (HTS) platforms to identify and evaluate potential neuraminidase inhibitors for therapeutic development, particularly for viral and bacterial infections.
• Glycobiology Research: Serves as a critical tool for studying sialic acid metabolism, glycosylation pathways, and the role of sialidases in cellular processes and disease states.
• Diagnostic Kit Development: Employed in the formulation of diagnostic kits for the detection and quantification of neuraminidase activity in clinical and research samples.
• Biochemical Probe Synthesis: Acts as a protected intermediate in the synthetic pathway for creating more complex sialic acid derivatives and labeled probes for imaging and detection.
• Quality Control in Biologics: Used in the biopharmaceutical industry to monitor and control the activity of enzymes used in manufacturing processes involving glycoproteins.

Basic Information

Product Name	4-Trifluoromethylumbelliferyl Tetra-o-Acetylated α-D-n-Acetylneuraminate Methyl Ester
CAS No.	195210-09-4
Molecular Formula	C31H35F3N2O17
Molecular Weight	764.61 g/mol
Synonyms	4-MU-α-(2,3,4,6-Tetra-O-acetyl-N-acetylneuraminyl)-methyl ester; 4-Trifluoromethylumbelliferyl-α-D-N-acetylneuraminate tetraacetate methyl ester; 4-TFMU-Neu5AcαMe tetraacetate; Fluorogenic neuraminidase substrate (tetraacetylated); α-N-Acetylneuraminyl 4-trifluoromethylumbelliferyl methyl ester peracetate; Sialidase substrate 4-TFMU; 4-Trifluoromethylumbelliferyl α-D-sialoside (peracetylated, methyl ester); CAS 195210-09-4
EINECS	Contact for details

Quality Control

Our 4-Trifluoromethylumbelliferyl Tetra-o-Acetylated α-D-n-Acetylneuraminate Methyl Ester is produced under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider under an inert atmosphere to maintain stability. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in alcohols

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.