Description

H-Met-Leu-Pna CAS NO 194612-51-6 is a high-purity synthetic peptide derivative, specifically a tripeptide chromogenic substrate. This compound is a critical reagent in biochemical and pharmaceutical research, valued for its role as a selective enzyme substrate. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in protease activity studies, enzyme kinetics, and high-throughput screening assays.

Application

• Protease & Enzyme Research: Acts as a specific chromogenic substrate for studying the activity and inhibition kinetics of various proteolytic enzymes.
• Diagnostic Assay Development: Used in the formulation of colorimetric diagnostic kits for detecting and quantifying specific enzyme activities in clinical samples.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors as potential drug candidates.
• Biochemical Pathway Analysis: A tool for investigating proteolytic cascades and signal transduction pathways in cellular and molecular biology.
• Quality Control in Biologics: Used to monitor the presence and activity of specific proteases during the production and purification of biopharmaceuticals.

Basic Information

Product Name	H-Met-Leu-Pna
CAS No.	194612-51-6
Molecular Formula	C20H30N4O5S
Molecular Weight	438.54 g/mol
Synonyms	H-Met-Leu-pNA; Methionyl-leucyl-p-nitroanilide; L-Methionyl-L-leucine 4-nitroanilide; N-(4-Nitrophenyl)-L-methionyl-L-leucinamide; Substrate for Proteases; Chromogenic Protease Substrate; pNA Substrate; Peptide p-nitroanilide
EINECS	Contact for details

Quality Control

Our H-Met-Leu-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, NMR and MS for structural confirmation, and specific tests for residual solvents and moisture. Certificates of Analysis (COA) detailing all specifications are provided to ensure compliance with research-grade standards and support your regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.