Description

Sulfo-NHS-LC-LC-Biotin is a heterobifunctional, water-soluble crosslinking reagent designed for efficient bioconjugation. Its core value lies in its extended spacer arm and amine-reactive N-hydroxysulfosuccinimide (Sulfo-NHS) ester, enabling the stable, covalent labeling of proteins, antibodies, and other biomolecules with biotin for detection or capture. This reagent is essential for researchers and manufacturers in proteomics, immunoassay development, and diagnostic kit production who require high-efficiency, site-specific biotinylation under aqueous conditions.

Application

• Antibody and Protein Biotinylation: For labeling primary antibodies for use in ELISA, Western blotting, and flow cytometry with streptavidin-based detection systems.
• Diagnostic Assay Development: As a key component in the manufacturing of lateral flow assays (LFAs) and other immunoassays that utilize a biotin-streptavidin signal amplification strategy.
• Affinity Purification Probe Preparation: For creating biotinylated ligands or capture molecules for use with streptavidin-coated beads or columns in pull-down experiments.
• Cell Surface Labeling: For tagging cell surface proteins or receptors with biotin for subsequent isolation or imaging studies.
• Microarray and Chip Fabrication: For the controlled immobilization of biomolecules onto streptavidin-functionalized biosensor surfaces and microarray slides.
• Nucleic Acid Probe Conjugation: For attaching biotin to oligonucleotides or other nucleic acid probes for hybridization-based detection applications.

Basic Information

Product Name	Sulfo-NHS-LC-LC-Biotin
CAS No.	194041-66-2
Molecular Formula	C26H41N5O10S2
Molecular Weight	655.8 g/mol
Synonyms	Sulfosuccinimidyl-6-(biotinamido)-6-hexanamido hexanoate; Biotin-LC-LC-Sulfo-NHS; Nε-(+)-Biotinyl-6-aminocaproyl-6-aminocaproic acid N-hydroxysulfosuccinimide ester; Sulfo-NHS-LC-LC-Biotin ester; EZ-Link Sulfo-NHS-LC-LC-Biotin; 6-((6-(Biotinamido)hexanoyl)amino)hexanoic acid sulfo-NHS ester; Biotin-XX sulfosuccinimidyl ester; Biotin (long chain) Sulfo-NHS Ester
EINECS	Contact for details

Quality Control

Our Sulfo-NHS-LC-LC-Biotin is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity and functional suitability. We adhere to ISO 9001 quality management standards, and our production environment is designed to meet the rigorous demands of diagnostic and life science research.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO, DMF
Functional Activity	Conforms (by biotinylation test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.