Description

Mmp Substrate Iii, Fluorogenic is a specialized, high-purity fluorogenic peptide substrate designed for the sensitive and continuous detection of matrix metalloproteinase (MMP) activity. This compound matters because it provides researchers with a critical tool for studying proteolytic processes involved in cancer metastasis, tissue remodeling, and inflammatory diseases. Life science researchers, pharmaceutical developers, and diagnostic kit manufacturers need it for high-throughput screening, enzyme kinetics studies, and the development of novel therapeutic and diagnostic assays.

Application

• Biochemical Research: Used as a key reagent for the in vitro fluorometric assay of matrix metalloproteinase (MMP) enzyme activity, particularly MMP-2, MMP-9, and related gelatinases.
• Drug Discovery & Development: Enables high-throughput screening (HTS) of potential MMP inhibitor compounds in pharmaceutical R&D pipelines.
• Cancer Biology Studies: Facilitates the investigation of tumor cell invasion, angiogenesis, and metastasis by monitoring MMP-mediated extracellular matrix degradation.
• Diagnostic Assay Development: Serves as a core component in developing fluorogenic diagnostic kits for detecting specific MMP levels in biological samples.
• Enzyme Kinetics & Characterization: Employed to determine kinetic parameters (Km, Vmax, kcat) and inhibition constants (Ki, IC50) for various MMP isoforms.
• Tissue Engineering & Wound Healing Research: Applied in studies examining the role of MMPs in tissue remodeling, repair, and regenerative medicine.

Basic Information

Product Name	Mmp Substrate Iii, Fluorogenic
CAS No.	193475-71-7
Molecular Formula	C34H46F2N8O13
Molecular Weight	812.78 g/mol
Synonyms	MMP Substrate III, Fluorogenic; Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2; (7-Methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-(3-[2,4-dinitrophenyl]-L-2,3-diaminopropionyl)-Ala-Arg-NH2; Fluorogenic MMP Substrate; MMP-2/MMP-9 Fluorogenic Substrate; Dnp-Peptide Substrate; Mca-PLGL-Dpa-AR-NH2; MMP Fluorogenic Peptide Substrate III
EINECS	Contact for details

Quality Control

Our Mmp Substrate Iii, Fluorogenic is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and mass spectrometry for identity confirmation. We provide comprehensive Certificates of Analysis (COA) detailing purity, peptide content, and performance data in standard enzymatic assays. Our quality commitment aligns with the standards expected for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Area %)
Peptide Content	≥ 70% (by weight)
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Conforms to reference in standard MMP assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.