Description

25-Hydroxy Vitamin D3 3,3'-Biotinylaminopropyl Ether is a sophisticated biochemical conjugate designed for advanced research and diagnostic applications. This compound is critical for developing highly sensitive and specific assays by leveraging the strong affinity of biotin for streptavidin. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and clinical diagnostics industries for applications such as immunoassay development, receptor binding studies, and targeted drug delivery systems.

Application

• Immunoassay Development: Used as a labeled tracer or capture reagent in competitive and sandwich ELISA formats for the precise quantification of Vitamin D metabolites.
• Diagnostic Kit Manufacturing: A key component in the production of high-sensitivity commercial test kits for measuring 25-Hydroxy Vitamin D levels in serum or plasma.
• Biochemical Research: Employed in receptor-ligand interaction studies, signal amplification systems, and cellular uptake mechanisms related to Vitamin D pathways.
• Affinity Purification: Facilitates the isolation and purification of specific antibodies or proteins through biotin-streptavidin chromatography.
• Drug Discovery: Serves as a tool compound for investigating targeted delivery mechanisms where biotin receptors are overexpressed.
• Bioconjugation Reference Standard: Acts as a model compound for developing and validating new bioconjugation techniques and linker chemistries.

Basic Information

Product Name	25-Hydroxy Vitamin D3 3,3'-Biotinylaminopropyl Ether
CAS No.	193278-59-0
Molecular Formula	C₄₀H₆₈N₄O₅S
Molecular Weight	717.06 g/mol
Synonyms	25-Hydroxycholecalciferol 3,3'-Biotinylaminopropyl Ether; Biotinylated 25-Hydroxy Vitamin D3; 25(OH)D3-Biotin Conjugate; Calcifediol Biotin Derivative; 25-Hydroxyvitamin D3 Biotin Label; Vitamin D Metabolite-Biotin Conjugate; 3,3'-Biotinylaminopropylether of 25-Hydroxy Vitamin D3; 25-Hydroxy Vitamin D3-Biotin
EINECS	Contact for details

Quality Control

Our 25-Hydroxy Vitamin D3 3,3'-Biotinylaminopropyl Ether is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high purity suitable for sensitive research and in-vitro diagnostic use. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance characteristics. We adhere to relevant quality management standards to support our global clientele in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep the primary container sealed in a secondary protective container. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; Sparingly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.