Description

5’-o-Dmtr-2’-Omeu-Methyl Phosphonamidite is a high-purity phosphoramidite monomer, a critical building block in the solid-phase synthesis of oligonucleotides. Its value lies in enabling the precise and efficient incorporation of modified nucleosides, which is essential for developing advanced therapeutic and diagnostic nucleic acid sequences. This product is primarily needed by biotechnology and pharmaceutical companies engaged in the research and production of antisense oligonucleotides, siRNAs, and aptamers, where sequence fidelity and modification control are paramount.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite reagent for the automated synthesis of modified DNA/RNA strands.
• Therapeutic Oligonucleotide Development: For the research and GMP-scale production of antisense, siRNA, and miRNA-based drugs requiring 2'-O-methyl modifications.
• Diagnostic Probe Synthesis: Manufacturing of labeled and modified nucleic acid probes for assays, hybridization, and next-generation sequencing platforms.
• Aptamer and Ribozyme Research: Construction of nuclease-resistant aptamers and catalytic nucleic acids with enhanced stability and specificity.
• Process Development & Scale-Up: Supporting process optimization and pilot-scale manufacturing for oligonucleotide active pharmaceutical ingredients (APIs).
• Academic & Contract Research: Enabling fundamental research in molecular biology, genetics, and biochemistry that utilizes custom synthetic oligonucleotides.

Basic Information

Product Name	5’-o-Dmtr-2’-Omeu-Methyl Phosphonamidite
CAS No.	191786-64-8
Molecular Formula	C41H51N4O9P
Molecular Weight	774.85 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-methyluridine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); 2'-O-Methyl-5'-O-DMT-uridine 3'-CE Phosphoramidite; 2'-O-Me-U Phosphoramidite; 2'-O-Methyluridine Phosphoramidite; DMT-2'-O-Methyluridine-CE Phosphoramidite; 2'-O-Methyl-5'-O-(4,4'-dimethoxytrityl)uridine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
EINECS	Contact for details

Quality Control

Our 5’-o-Dmtr-2’-Omeu-Methyl Phosphonamidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for demanding oligonucleotide synthesis applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry. Certificates of Analysis (COA) with detailed specifications are provided and can be tailored to meet specific customer or regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, cool place. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures should be avoided to maintain stability and reactivity.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, DCM

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.