Description

H-Ile-Amc Tfa is a specialized protected amino acid derivative, specifically the TFA salt of H-Isoleucine-7-amido-4-methylcoumarin. This compound serves as a critical fluorogenic substrate for the sensitive detection and study of protease enzyme activity. It is primarily utilized by researchers and manufacturers in the life sciences and pharmaceutical development sectors. Its application is essential for high-throughput screening, enzyme kinetics studies, and biochemical assay development.

Application

• Protease Enzyme Assays: Acts as a fluorogenic substrate for various proteolytic enzymes, including chymotrypsin, elastase, and subtilisin.
• Biochemical Research: Used in kinetic studies to determine enzyme activity, inhibition constants (Ki), and catalytic mechanisms.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify and characterize novel protease inhibitors.
• Diagnostic Development: Serves as a key component in developing sensitive fluorometric diagnostic tests for pathological conditions involving protease imbalances.
• Peptide Chemistry: Used as a building block or specialized reagent in peptide synthesis and modification research.
• Quality Control in Biotechnology: Applied in the QC testing of enzyme preparations and biopharmaceutical products to ensure batch-to-batch consistency and activity.

Basic Information

Product Name	H-Ile-Amc Tfa
CAS No.	191723-68-9
Molecular Formula	C₂₀H₂₅F₃N₂O₅
Molecular Weight	430.42 g/mol
Synonyms	H-Isoleucine-7-amido-4-methylcoumarin Trifluoroacetate Salt; Ile-AMC TFA; L-Isoleucyl-7-amido-4-methylcoumarin TFA; AMC-Ile TFA; 7-Amido-4-methylcoumarin-Ile TFA; Protease Substrate Ile-AMC; Fluorogenic Substrate for Protease; N-Isoleucyl-7-amino-4-methylcoumarin Trifluoroacetate
EINECS	Contact for details

Quality Control

Every batch of H-Ile-Amc Tfa is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure high purity and batch-to-batch consistency suitable for sensitive research and development applications. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity, identity, and other critical parameters. Our quality commitment aligns with the standards expected in pharmaceutical and biotechnology research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, keep desiccated under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Specific Rotation	Contact for details
Fluorogenic Activity	Functional in standard assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.