Description

H-Thr-Amc CAS NO 191723-66-7 is a specialized protected amino acid derivative, specifically L-Threonine coupled with a 7-amino-4-methylcoumarin (AMC) fluorophore. This compound is a critical building block and biochemical tool, valued for its role in advanced peptide synthesis and enzymatic research. It is primarily utilized by pharmaceutical R&D teams, peptide synthesis specialists, and diagnostic assay developers for creating fluorescently labeled peptides and substrates.

Application

• Fluorogenic Peptide Substrate Synthesis: Key component for synthesizing sensitive substrates used to measure protease and peptidase enzyme activity in high-throughput screening (HTS) and biochemical assays.
• Advanced Peptide Chemistry: Serves as a protected, fluorescently tagged amino acid building block in solid-phase and solution-phase peptide synthesis for research and diagnostic applications.
• Protease Research & Drug Discovery: Enables the study of enzyme kinetics, inhibition, and mechanism of action for various proteolytic enzymes, supporting target validation and lead optimization.
• Diagnostic Assay Development: Used in the development of sensitive fluorescent diagnostic kits and assays for clinical and research laboratories.
• Bioconjugation & Probe Design: Acts as a versatile intermediate for creating custom fluorescent probes and bioconjugates for cellular imaging and biomolecular interaction studies.

Basic Information

Product Name	H-Thr-Amc
CAS No.	191723-66-7
Molecular Formula	C₁₄H₁₆N₂O₅
Molecular Weight	292.29 g/mol
Synonyms	L-Threonine 7-amido-4-methylcoumarin; H-Thr-AMC; Thr-AMC; L-Thr-AMC; 7-Amino-4-methylcoumarin-L-threonine amide; N-(7-Amino-4-methyl-2-oxo-2H-chromen-3-yl)-L-threoninamide; Fluorogenic Threonine Substrate; AMC-Threonine derivative
EINECS	Contact for details

Quality Control

Our production of H-Thr-Amc adheres to stringent quality protocols to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive); keep the container tightly sealed in a desiccated environment to prevent degradation. For long-term storage, consider storing at -20°C under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.