Description

H-Gly-Gly-Amc Hcl CAS NO 191723-65-6 is a protected dipeptide derivative, specifically the hydrochloride salt of H-Gly-Gly-7-amido-4-methylcoumarin. This compound is a critical fluorogenic substrate used extensively in biochemical research and diagnostic assay development. It serves as a highly sensitive tool for the detection and quantification of specific protease activity, particularly aminopeptidases and dipeptidyl peptidases. Key industries that rely on this high-purity biochemical include pharmaceutical R&D, academic research institutions, and in vitro diagnostic (IVD) kit manufacturers.

Application

• Protease Activity Assays: Primary substrate for continuous fluorometric assays to measure the kinetic activity of aminopeptidases like DPP-IV (CD26) and other dipeptidyl peptidases.
• Enzyme Inhibition Studies: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery programs targeting diabetes, cancer, and immune disorders.
• Diagnostic Reagent Manufacturing: A key component in the formulation of clinical diagnostic kits for measuring specific enzyme levels in serum or plasma samples.
• Biochemical Research: Essential tool for studying enzyme kinetics, specificity, and mechanism of action in academic and industrial laboratory settings.
• Cell-Based Assays: Employed in live-cell imaging and flow cytometry to detect and localize protease activity within cellular systems.
• Quality Control in Biologics: Used to monitor the presence and activity of host cell proteases during the biomanufacturing process of therapeutic proteins.

Basic Information

Product Name	H-Gly-Gly-Amc Hcl
CAS No.	191723-65-6
Molecular Formula	C₁₅H₁₈N₄O₅ • HCl
Molecular Weight	370.79 g/mol (Free base: 334.33 g/mol)
Synonyms	Gly-Gly-AMC hydrochloride; H-Gly-Gly-7-Amido-4-methylcoumarin hydrochloride; Glycyl-glycyl 7-amido-4-methylcoumarin hydrochloride; DPP-IV Fluorogenic Substrate; DPPIV Substrate Gly-Pro-AMC related dipeptide; Aminopeptidase Substrate; 7-Amido-4-methylcoumarin derivative; H-Gly-Gly-AMC • HCl
EINECS	Contact for details

Quality Control

Every batch of H-Gly-Gly-Amc Hcl is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and performance consistency suitable for sensitive research and diagnostic applications. Our standard quality commitment includes comprehensive analysis by HPLC, NMR, and MS. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity (typically ≥95% by HPLC), and confirming the absence of interfering impurities.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm
Fluorogenic Activity	Functional performance verified vs. control

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.