Description

Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-Oh is a specialized, sequence-specific fluorogenic peptide substrate designed for the detection and study of caspase activity, particularly caspase-3 and related enzymes. This compound matters for its critical role in biochemical research and drug discovery, enabling precise measurement of protease activity in apoptosis and other cellular processes. It is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors who require reliable tools for enzyme kinetics and high-throughput screening assays.

Application

• Biochemical Research: As a fluorogenic substrate for studying the kinetics and inhibition of caspases, key enzymes in programmed cell death (apoptosis).
• Pharmaceutical Development: Used in high-throughput screening (HTS) assays to identify and characterize novel therapeutic compounds that modulate caspase activity.
• Cell Biology Studies: Employed to detect and quantify caspase activation in cell cultures and tissue samples during apoptosis research.
• Diagnostic Reagent Manufacturing: A key component in the formulation of test kits for clinical and research laboratories focused on cell death pathways.
• Quality Control (QC) Testing: Used in biopharmaceutical QC to monitor the presence and activity of caspases in production processes.

Basic Information

Product Name	Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-Oh
CAS No.	189696-01-3
Molecular Formula	C₅₃H₆₈N₁₂O₁₈
Molecular Weight	1161.17 g/mol
Synonyms	Caspase-3 Substrate I (Mca-DEVDAPK(Dnp)-OH); Mca-DEVDAPK(Dnp)-OH; (7-Methoxycoumarin-4-yl)acetyl-Tyr-Val-Ala-Asp-Ala-Pro-Lys(2,4-dinitrophenyl)-OH; Caspase 3 Fluorogenic Substrate; Ac-DEVDAPK(Dnp)-OH; Caspase-3/7 Substrate; Fluorogenic Caspase-3 Substrate; ApoAlert Caspase-3 Fluorescent Assay Substrate
EINECS	Contact for details

Quality Control

Our Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-Oh is produced under stringent conditions to ensure the highest standards of purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substances is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.