Description

Ac-Leu-Glu-Val-Asp-Pna CAS NO 189684-49-9 is a high-purity, chromogenic peptide substrate designed for biochemical research and diagnostic applications. This compound serves as a critical tool for the specific detection and quantification of caspase-3 and related protease activity, which is fundamental in apoptosis (programmed cell death) studies. It is essential for researchers and manufacturers in the fields of pharmaceutical development, oncology research, and clinical diagnostics who require reliable and sensitive reagents for enzyme activity assays.

Application

• Caspase-3 Activity Assays: Primary substrate for in vitro fluorometric or colorimetric detection of caspase-3 enzyme activity, a key marker of apoptosis.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and evaluate potential therapeutic compounds that modulate apoptotic pathways.
• Biochemical Research: Fundamental reagent for studying the mechanisms of apoptosis, cell signaling pathways, and protease function in academic and industrial laboratories.
• Diagnostic Kit Development: A key component in the formulation of diagnostic kits for measuring caspase activity in cell lysates and tissue samples.
• Oncology Research: Enables the assessment of chemotherapeutic agent efficacy by measuring the induction of apoptosis in cancer cell lines.
• Quality Control in Biopharma: Employed in the quality control of biopharmaceutical processes where apoptosis monitoring is critical.

Basic Information

Product Name	Ac-Leu-Glu-Val-Asp-Pna
CAS No.	189684-49-9
Molecular Formula	C29H41N7O11
Molecular Weight	663.68 g/mol
Synonyms	Ac-LEVD-pNA; Ac-Leu-Glu-Val-Asp-p-Nitroanilide; N-Acetyl-Leu-Glu-Val-Asp-p-nitroanilide; Caspase-3 Substrate (Ac-LEVD-pNA); Ac-L-E-V-D-pNA; Apoptosis Substrate; N-Acetyl-L-leucyl-L-α-glutamyl-L-valyl-L-α-aspartic acid 4-nitroanilide; L-E-V-D Protease Substrate
EINECS	Contact for details

Quality Control

Our Ac-Leu-Glu-Val-Asp-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, MS for identity confirmation, and NMR for structural verification, to ensure it meets the high standards required for sensitive research applications. A detailed Certificate of Analysis (COA) is provided with every shipment, guaranteeing traceability, consistency, and reliability for your critical work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO or DMF
Specific Optical Rotation	Contact for details
Peptide Content	≥ 70.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.