Description

Biotin-PEG5-NH-Boc is a high-purity, heterobifunctional polyethylene glycol (PEG) linker featuring a biotin moiety and a Boc-protected amine. This compound is engineered to facilitate the controlled conjugation of biomolecules, enabling precise biotinylation for downstream affinity capture and detection applications. It is an essential intermediate for researchers and manufacturers in the fields of bioconjugation, proteomics, and diagnostic assay development, where reliable linker chemistry is critical.

Application

• Bioconjugation Reagent: Used for the site-specific biotinylation of peptides, proteins, antibodies, and other biomolecules.
• Affinity Chromatography Ligand Synthesis: A key building block for creating immobilized biotin ligands on solid supports like agarose or magnetic beads for streptavidin/avidin-based purification.
• Diagnostic Assay Development: Enables the labeling of probes and detection molecules in ELISA, lateral flow assays, and other immunoassay platforms.
• Drug Delivery System Fabrication: Serves as a functional component in the synthesis of PEGylated drug conjugates and targeted nanoparticle systems.
• Proteomics & Molecular Biology: Facilitates pull-down assays, protein interaction studies, and cellular imaging through biotin-streptavidin technology.
• Surface Functionalization: Used to modify surfaces (e.g., chips, slides, sensors) with biotin groups for biosensor and microarray applications.

Basic Information

Product Name	Biotin-PEG5-NH-Boc
CAS No.	189209-28-7
Molecular Formula	C31H57N3O10S
Molecular Weight	663.87 g/mol
Synonyms	Biotin-PEG5-amine-Boc; Boc-NH-PEG5-Biotin; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(14-((tert-butoxycarbonyl)amino)-3,6,9,12-tetraoxatetradecyl)pentanamide; Biotinamidopentane-N-Boc-pentaoxyethyleneamine; Biotin-Pentaethylene glycol-amine-Boc; Biotinylated PEG linker, Boc-protected; PEG5-Biotin-NH-Boc
EINECS	Contact for details

Quality Control

Our Biotin-PEG5-NH-Boc is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment, NMR and MS for structural confirmation, and KF titration for moisture content. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to ensure traceability and compliance with research and cGMP-grade material standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.