Description

Ac-Asp-Glu-Asp(Edans)-Glu-Glu-Abu-L-Lactoyl-Ser-Lys(Dabcyl)-Nh2 is a specialized, high-purity synthetic peptide substrate designed for advanced biochemical research and diagnostic applications. This compound matters because it serves as a critical tool for studying and detecting specific protease activity, particularly in complex biological systems. It is primarily needed by researchers and developers in the fields of pharmaceutical discovery, clinical diagnostics, and proteomics who require precise and sensitive assay components.

Application

• Fluorogenic Protease Assays: As a key substrate in FRET (Förster Resonance Energy Transfer)-based assays for monitoring enzyme kinetics and inhibitor screening.
• Drug Discovery Research: For high-throughput screening (HTS) platforms to identify and characterize novel therapeutic compounds targeting specific proteases.
• Diagnostic Kit Development: Used in the formulation of sensitive and specific diagnostic tests for diseases where protease biomarkers are relevant.
• Biochemical Pathway Analysis: Enables the study of protease involvement in cellular processes like apoptosis, signal transduction, and inflammation.
• Enzyme Characterization: Assists in determining the substrate specificity, catalytic efficiency, and optimal conditions for target proteases.
• Academic and Institutional Research: A vital reagent for fundamental research in biochemistry, molecular biology, and cell biology laboratories worldwide.

Basic Information

Product Name	Ac-Asp-Glu-Asp(Edans)-Glu-Glu-Abu-L-Lactoyl-Ser-Lys(Dabcyl)-Nh2
CAS No.	188530-20-3
Molecular Formula	C73H98N16O28S2
Molecular Weight	1687.8 g/mol
Synonyms	FRET Substrate for Caspase-3; Ac-DEVD-EDANS/DABCYL Substrate; Caspase-3 Fluorogenic Substrate I; Ac-Asp-Glu-Val-Asp-EDANS/DABCYL; Caspase-3 Substrate (Ac-DEVD-AMC related analog); EDANS-DABCYL FRET Peptide; Protease Detection Peptide; Synthetic Fluorogenic Peptide Substrate
EINECS	Contact for details

Quality Control

Every batch of our synthetic peptide is manufactured under strict quality management and undergoes comprehensive analytical testing to ensure identity, purity, and performance. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes data from HPLC, MS, and amino acid analysis. Our production adheres to standards suitable for research and in-vitro diagnostic use, ensuring reliable and reproducible results for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70% (by weight)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO or aqueous buffer
Functional Activity	Tested in relevant assay system

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.