Description

2-(n-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Amino)-2-Deoxyglucose is a fluorescently labeled glucose analog, a critical tool compound for probing cellular glucose uptake and metabolism. Its core value lies in its ability to act as a non-metabolizable or poorly metabolized tracer, enabling real-time, high-sensitivity visualization and quantification of glucose transport activity in living systems. This product is essential for researchers and developers in cell biology, oncology, diabetes research, and drug discovery, where understanding metabolic pathways is paramount.

Application

• Cellular Glucose Uptake Assays: Direct visualization and quantification of glucose transporter (GLUT) activity in live cells using fluorescence microscopy or flow cytometry.
• Cancer Metabolism Research: Investigating the Warburg effect and altered glycolytic flux in tumor cell lines and primary tissues.
• Drug Discovery Screening: Serving as a key reagent in high-throughput screens for compounds that modulate glucose metabolism, targeting metabolic diseases and oncology.
• Neuroscience Studies: Probing astrocyte-neuron metabolic coupling and brain energy metabolism under physiological and pathological conditions.
• Diabetes and Insulin Signaling Research: Assessing the impact of insulin and other hormones on glucose transport in adipocytes, muscle cells, and other insulin-sensitive tissues.
• Microbial Metabolism: Studying sugar transport mechanisms in bacteria and yeast.

Basic Information

Product Name	2-(n-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Amino)-2-Deoxyglucose
CAS No.	186689-07-6
Molecular Formula	C14H16N4O8
Molecular Weight	368.30 g/mol
Synonyms	2-NBDG; 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxy-D-glucose; NBDG; 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose; Fluorescent D-glucose analog; 2-(7-Nitro-2,1,3-benzoxadiazol-4-ylamino)-2-deoxy-D-glucose; Glucose Uptake Probe
EINECS	Contact for details

Quality Control

Our 2-NBDG is manufactured under strict quality protocols to ensure batch-to-batch consistency and performance reliability for sensitive research applications. Each lot is characterized by HPLC, NMR, and mass spectrometry to confirm identity and high chemical purity. We provide a comprehensive Certificate of Analysis (COA) with each shipment, detailing purity, fluorescence properties, and absence of critical impurities. Our quality system supports research requiring GMP/GLP-compliant materials.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccant and under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Fluorescence Properties	Ex/Em ~465/540 nm (in aqueous buffer)
Biological Activity	Suitable for cell-based glucose uptake assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.