Description

n-Benzoyl-2'-Deoxy-5'-o-Dmt-2'-Fluoro-5-Methylcytidine 3'-Ce Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. Its value lies in enabling the precise and efficient incorporation of modified 5-methylcytidine analogs into synthetic DNA strands, which is essential for advanced research and therapeutic development. This product is primarily needed by biotechnology and pharmaceutical companies engaged in antisense drug discovery, siRNA development, and the creation of diagnostic probes where sequence-specific modification and stability are paramount.

Application

• Antisense Oligonucleotide (ASO) Synthesis: For the production of therapeutic oligonucleotides targeting specific mRNA sequences.
• Small Interfering RNA (siRNA) Manufacture: As a key monomer for introducing stabilizing 2'-fluoro and 5-methyl modifications into siRNA strands to enhance nuclease resistance and potency.
• Diagnostic Probe Development: Used in synthesizing labeled or modified DNA probes for PCR, microarrays, and fluorescence in situ hybridization (FISH) applications.
• Research-Grade Oligo Synthesis: For academic and industrial research into nucleic acid chemistry, structure-function relationships, and novel oligonucleotide-based technologies.
• Aptamer Production: In the synthesis of chemically modified DNA aptamers with improved binding affinity and stability for therapeutic or sensing applications.
• Synthesis of Modified Primers: For creating specialized PCR primers with internal modifications to study epigenetic markers like DNA methylation.

Basic Information

Product Name	n-Benzoyl-2'-Deoxy-5'-o-Dmt-2'-Fluoro-5-Methylcytidine 3'-Ce Phosphoramidite
CAS No.	182495-83-6
Molecular Formula	C47H53FN5O8P
Molecular Weight	865.93 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxy-2'-fluoro-5-methylcytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite, N4-Benzoyl-5'-O-DMT-2'-fluoro-5-methyl-2'-deoxycytidine-3'-CE phosphoramidite; 2'-Fluoro-5-methyl-dC(Bz) Phosphoramidite; 5-Me-2'-F-dC Phosphoramidite; 2'-F-5-Me-dC(Bz) CE Phosphoramidite; 5-Methyl-2'-deoxy-2'-fluorocytidine phosphoramidite (N4-benzoyl, 5'-DMT); 2'-Fluoro-5-methylcytidine CE phosphoramidite
EINECS	Contact for details

Quality Control

Our n-Benzoyl-2'-Deoxy-5'-o-Dmt-2'-Fluoro-5-Methylcytidine 3'-Ce Phosphoramidite is manufactured under strict quality systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is rigorously tested using advanced analytical techniques, including HPLC and 31P NMR, to confirm identity, purity, and coupling efficiency. A comprehensive Certificate of Analysis (COA) detailing assay, moisture content, and critical impurity profiles is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will lead to decomposition and reduced coupling efficiency. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Assay (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Coupling Efficiency Test	≥ 98.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.