Description

Fmoc-Asp(Edans)-Oh is a specialized, fluorescently labeled amino acid derivative used in advanced peptide synthesis and biochemical research. This compound is critical for researchers developing fluorescence resonance energy transfer (FRET)-based assays and protease activity studies. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and biotechnology companies focused on enzymology, peptide chemistry, and diagnostic probe development.

Application

• FRET-Based Protease Assays: Serves as a key component in substrates for monitoring protease (e.g., caspase, HIV protease) activity, where cleavage separates the Edans fluorophore from the Dabcyl quencher.
• Peptide Synthesis & Labeling: Used in solid-phase peptide synthesis (SPPS) to incorporate a fluorescent reporter (Edans) at specific aspartic acid residues within custom peptide sequences.
• Biochemical Probe Development: Enables the creation of sensitive fluorescent probes for real-time monitoring of enzymatic reactions and protein-protein interactions.
• Drug Discovery Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel enzyme inhibitors for therapeutic targets.
• Diagnostic Reagent Formulation: A building block for synthesizing components used in clinical diagnostic kits for detecting specific enzyme levels.
• Academic & Industrial Research: Supports fundamental research in biochemistry, cell biology, and medicinal chemistry involving fluorescence detection methodologies.

Basic Information

Product Name	Fmoc-Asp(Edans)-Oh
CAS No.	182253-73-2
Molecular Formula	C₃₄H₃₃N₃O₉S
Molecular Weight	651.71 g/mol
Synonyms	Fmoc-Asp(EDANS)-OH; Fmoc-L-Aspartic acid 5-((2-aminoethyl)amino)naphthalene-1-sulfonyl derivative; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-aspartic acid 5-((2-aminoethyl)amino)naphthalene-1-sulfonamide; Fmoc-Asp(Edans); Fmoc-Aspartic acid(Edans); EDANS-labeled Fmoc-Asp-OH; Fmoc-Asp-EDANS; Nα-Fmoc-Nβ-(5-((2-aminoethyl)amino)naphthalene-1-sulfonyl)-L-aspartic acid
EINECS	Contact for details

Quality Control

Our Fmoc-Asp(Edans)-Oh is manufactured under strict quality-controlled conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to ISO 9001 quality management standards, and our production processes are designed to meet the rigorous demands of pharmaceutical and biotechnology research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.