Description

25-Hydroxycholesterol-(26,27-3H) is a high-specific-activity, tritium-labeled derivative of the endogenous oxysterol, 25-hydroxycholesterol. This radiolabeled compound is an essential tool for tracing and quantifying metabolic pathways, receptor binding studies, and enzyme activity assays in biochemical research. It is primarily utilized by pharmaceutical R&D teams, academic researchers, and diagnostic developers in the fields of lipid metabolism, cholesterol homeostasis, and nuclear receptor signaling.

Application

• Metabolic Pathway Tracing: Used as a radiolabeled tracer to study the biosynthesis, transport, and degradation of cholesterol and its derivatives in cellular and animal models.
• Receptor Binding Assays: Critical for competitive binding studies and saturation analysis to characterize interactions with nuclear receptors like LXR (Liver X Receptor).
• Enzyme Activity Studies: Employed as a substrate to measure the activity of enzymes involved in oxysterol metabolism, such as sulfotransferases and hydroxylases.
• Drug Discovery & Development: Supports high-throughput screening (HTS) and mechanism-of-action studies for therapeutic agents targeting lipid-related diseases (e.g., atherosclerosis, NAFLD).
• Biomarker Research: Facilitates the development of sensitive analytical methods (e.g., LC-MS/MS) for quantifying oxysterols as potential disease biomarkers.
• Academic & Institutional Research: Fundamental research tool for investigating the role of oxysterols in immune function, cancer biology, and neurodegenerative disorders.

Basic Information

Product Name	25-Hydroxycholesterol-(26,27-3H)
CAS No.	179383-16-5
Molecular Formula	C₂₇H₄₄O₂ (³H₂)
Molecular Weight	~402.6 g/mol (unlabeled)
Synonyms	(25R)-26-Hydroxycholesterol-(26,27-3H); Cholest-5-ene-3β,25-diol-(26,27-3H); 25-Hydroxycholesterol tritiated; 25-HC-(26,27-3H); 25-OH-Cholesterol-(26,27-3H); [26,27-3H]-25-Hydroxycholesterol; Oxysterol (³H-labeled); LXR ligand (tritiated)
EINECS	Contact for details

Quality Control

Every batch of 25-Hydroxycholesterol-(26,27-3H) is manufactured and analyzed under strict quality systems. Radiochemical purity and specific activity are determined by validated methods including HPLC with radiometric detection. We provide comprehensive Certificates of Analysis (COA) detailing purity, concentration, solvent, and specific activity (typically ranging from 40-80 Ci/mmol) to ensure reproducible and reliable results for your critical research. Our quality commitment supports compliance with GLP (Good Laboratory Practice) standards.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C. For long-term storage, solutions are recommended to be kept at -70°C to -80°C under inert atmosphere to minimize radiolysis and chemical degradation. The product is strictly light-sensitive (protect from all light exposure).

Specification

Item	Specification
Appearance	Solution in ethanol or toluene (colorless to pale yellow)
Chemical Purity (HPLC)	≥ 95%
Radiochemical Purity	≥ 97%
Specific Activity	40 - 80 Ci/mmol (1.48 - 2.96 TBq/mmol)
Concentration	0.1 - 1.0 mCi/mL (3.7 - 37 MBq/mL)
Identification (HPLC, MS)	Conforms to reference standard
Solvent	Ethanol or Toluene (as specified)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.