Description

5-(3-Amino-1-Propyn-1-Yl)-2'-Deoxyuridine 5'-(Tetrahydrogen Triphosphate) is a modified nucleoside triphosphate analog, a critical building block for advanced molecular biology and biochemistry research. This compound is essential for applications requiring the site-specific incorporation of functionalized nucleotides, enabling precise labeling and probing of nucleic acid structures. It is primarily utilized by researchers and manufacturers in the fields of next-generation sequencing (NGS), polymerase chain reaction (PCR) probe development, and structural biology studies.

Application

• Next-Generation Sequencing (NGS): Used as a modified dUTP analog for sequencing-by-synthesis platforms to introduce specific functionalities or termination points.
• PCR Probe Synthesis: Enables the enzymatic incorporation of labeled or tagged nucleotides for the creation of highly specific detection probes in diagnostic assays.
• Structural Biology & Biophysics: Acts as a substrate for polymerases in studies investigating enzyme kinetics, fidelity, and nucleic acid-protein interactions.
• Nucleic Acid Labeling: Serves as a precursor for the synthesis of fluorescently or chemically tagged nucleotides for imaging and detection applications.
• Antiviral & Anticancer Research: Investigated as a potential chain terminator or modulator in the development of nucleoside-based therapeutic agents.
• DNA Nanotechnology: Utilized in the assembly of functionalized DNA nanostructures where precise chemical modification is required.

Basic Information

Product Name	5-(3-Amino-1-Propyn-1-Yl)-2'-Deoxyuridine 5'-(Tetrahydrogen Triphosphate)
CAS No.	179101-49-6
Molecular Formula	C₁₃H₁₉N₄O₁₄P₃
Molecular Weight	572.23 g/mol
Synonyms	5-(3-Aminoprop-1-yn-1-yl)-2'-deoxyuridine-5'-triphosphate; Aminoalkynyl-dUTP; 5-(3-Aminopropynyl)-2'-deoxyuridine triphosphate; 5-Propargylamino-dUTP; Modified dUTP Triphosphate; NTP Analog; 5-Substituted dUTP; CAS 179101-49-6
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical adheres to stringent quality protocols. Each batch is characterized and tested to ensure identity, purity, and functional performance suitable for sensitive research applications. Quality is verified via advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, impurity profile, and functional assay data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Functional Assay (Enzymatic Incorporation)	≥ 90% incorporation efficiency
Solubility	Soluble in water or aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.