Description

Nbd-Pe [n-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)-1,2-Dihexadecanoyl-Sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt] is a high-purity, fluorescently labeled phospholipid derivative, widely recognized as a critical tool in membrane biophysics and cell biology research. Its core value lies in its ability to incorporate into lipid bilayers and serve as a highly sensitive fluorescent probe for studying membrane dynamics, fusion, and trafficking. This compound is essential for researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic sectors who require reliable, high-performance reagents for advanced fluorescence-based assays and imaging.

Application

• Membrane Fluorescence Labeling: Primary application for incorporating into liposomes, lipid bilayers, and cellular membranes to study structure, fluidity, and phase behavior.
• Lipid Trafficking & Fusion Assays: Used as a fluorescent tracer to monitor intracellular lipid transport, vesicle fusion, and endocytic pathways.
• Model Membrane Studies: Key component in constructing artificial membranes (e.g., giant unilamellar vesicles, supported lipid bilayers) for biophysical research.
• Fluorescence Resonance Energy Transfer (FRET): Acts as an efficient donor or acceptor dye in FRET pairs to investigate molecular interactions at membrane interfaces.
• Cell Staining & Imaging: Employed for selective staining of cellular membranes in fluorescence microscopy (e.g., confocal, TIRF) to visualize membrane domains.
• Drug Delivery Research: Utilized to label and track liposomal drug carriers to evaluate their stability, targeting, and cellular uptake efficiency.
• Sensor Development: Serves as a signaling component in the development of biosensors for detecting membrane-associated enzymes or environmental changes.
• Quality Control in Liposome Production: Acts as a process control marker to assess the encapsulation efficiency and integrity of manufactured lipid nanoparticles.

Basic Information

Product Name	Nbd-Pe [n-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)-1,2-Dihexadecanoyl-Sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt]
CAS No.	178119-00-1
Molecular Formula	C46H81N4O12P · C6H15N
Molecular Weight	~ 959.15 g/mol (Free acid), ~ 1058.31 g/mol (as Triethylammonium Salt)
Synonyms	NBD-PE; 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl); N-(7-Nitro-2,1,3-benzoxadiazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine; Fluorescent PE; NBD Phosphatidylethanolamine; NBD-DPPE; 16:0 NBD PE; NBD labeled DPPE; NBD-C16-HPC; NBD Fluorescent Lipid
EINECS	Contact for details

Quality Control

Our Nbd-Pe is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment, spectroscopic verification (UV-Vis, Fluorescence), and identity confirmation (MS, NMR). We provide a detailed Certificate of Analysis (COA) with every shipment, documenting critical parameters such as purity, fluorescence properties, and solvent content. Our quality system is designed to support research and development requiring reliable and traceable fluorescent probes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep the container sealed under an inert atmosphere (e.g., argon or nitrogen) to maintain stability. Allow the vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with structure
Purity (HPLC)	≥ 98.0%
Extinction Coefficient (ε)	~21,000 M-1cm-1 at ~460 nm in methanol
Fluorescence Emission	~534 nm (in lipid membranes, excitation ~460 nm)
Solvent Residue (GC)	Meet ICH guidelines
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.