Description

n-(5-Bromo-3-Indoxyl)-L-Alaninamide, Trifluoroacetate Salt is a specialized chromogenic substrate used in enzymatic assays and diagnostic research. This compound is critical for the sensitive detection and quantification of specific hydrolase enzymes, such as proteases, due to its ability to produce a distinct, measurable color change upon cleavage. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and in vitro diagnostics (IVD) industries for applications requiring precise biochemical analysis.

Application

• Enzymatic Substrate for Protease Assays: Used as a chromogenic substrate for the detection and kinetic study of various proteolytic enzymes.
• In Vitro Diagnostic (IVD) Reagents: A key component in the formulation of diagnostic kits for clinical testing and medical research.
• Biochemical Research: Employed in academic and industrial laboratories for studying enzyme mechanisms, inhibition, and activity profiling.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify and characterize potential therapeutic enzyme inhibitors.
• Histochemistry & Cell Biology: Applied in staining techniques to localize enzyme activity within tissues and cells.
• Quality Control in Biomanufacturing: Serves as a tool for monitoring the activity and purity of enzyme preparations during production.

Basic Information

Product Name	n-(5-Bromo-3-Indoxyl)-L-Alaninamide, Trifluoroacetate Salt
CAS No.	177966-70-0
Molecular Formula	C₁₃H₁₄BrN₃O₃ • C₂HF₃O₂
Molecular Weight	Contact for details
Synonyms	5-Bromo-3-indolyl-L-alaninamide trifluoroacetate; L-Alaninamide, N-(5-bromo-3-indolyl)-, trifluoroacetate; 5-Bromo-3-indoxyl-L-alaninamide TFA salt; Chromogenic protease substrate; 177966-70-0; BIA TFA salt
EINECS	Contact for details

Quality Control

Our n-(5-Bromo-3-Indoxyl)-L-Alaninamide, Trifluoroacetate Salt is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be kept in a dry environment. For long-term storage, consider desiccant use.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.