Description

4-Nitrophenyl2-Acetamido-2-Deoxy-4-o-[2-o-(A-L-Fucopyranosyl)-B-D-Galactopyranosyl]-B-D-Glucopyranoside is a specialized synthetic glycoside derivative, primarily valued as a high-purity chromogenic substrate for enzymatic research and diagnostic applications. Its core value lies in enabling precise, colorimetric detection and quantification of specific glycosidase enzyme activities, which is critical for biochemical pathway analysis and assay development. This compound is essential for research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in carbohydrate chemistry, glycobiology, and the development of novel therapeutic and diagnostic tools.

Application

• Enzymatic Substrate for Glycosidases: Acts as a specific chromogenic substrate for α-L-fucosidase and related exoglycosidases in biochemical assays.
• Diagnostic Reagent Manufacturing: Used in the production of clinical diagnostic kits for the detection of enzyme deficiencies, such as fucosidosis.
• Biochemical Research & Pathway Analysis: Facilitates the study of carbohydrate metabolism, glycoprotein processing, and lysosomal storage disorders in research laboratories.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify inhibitors or activators of specific glycosylhydrolase enzymes.
• Quality Control in Biologics: Used to monitor and characterize glycosidase activities during the production and purification of biopharmaceuticals.
• Teaching & Reference Standard: Serves as a purified standard in academic settings for teaching enzymology principles and in quality control labs.

Basic Information

Product Name	4-Nitrophenyl2-Acetamido-2-Deoxy-4-o-[2-o-(A-L-Fucopyranosyl)-B-D-Galactopyranosyl]-B-D-Glucopyranoside
CAS No.	177855-99-1
Molecular Formula	C32H46N2O20
Molecular Weight	778.71 g/mol
Synonyms	p-Nitrophenyl-α-L-fucopyranosyl-(1→2)-β-D-galactopyranosyl-(1→4)-2-acetamido-2-deoxy-β-D-glucopyranoside; 4-Nitrophenyl O-α-L-Fucopyranosyl-(1→2)-O-β-D-Galactopyranosyl-(1→4)-2-acetamido-2-deoxy-β-D-glucopyranoside; Fucα(1-2)Galβ(1-4)GlcNAcβ-pNP; pNP-Fucα1-2Galβ1-4GlcNAc; Chromogenic Fucosidase Substrate; α-L-Fucosidase Substrate; Synthetic Trisaccharide Derivative
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl2-Acetamido-2-Deoxy-4-o-[2-o-(A-L-Fucopyranosyl)-B-D-Galactopyranosyl]-B-D-Glucopyranoside is manufactured under strict quality management systems to ensure high purity and batch-to-batch consistency, critical for sensitive enzymatic assays. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assay conditions. We adhere to relevant guidelines for research and diagnostic-grade chemicals, ensuring reliability for your most demanding applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This compound is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake. For extended storage, consider desiccant in the storage environment.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Rotation	Contact for details
Enzymatic Activity	Contact for details
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.