Description

Ac-Phe-Arg-Amc Hcl is a fluorogenic peptide substrate designed for the sensitive detection and quantification of protease activity. This compound is of critical importance in biochemical research and pharmaceutical development, where precise enzyme kinetics are measured. It is primarily needed by researchers and quality control laboratories in the life sciences, diagnostics, and drug discovery sectors for studying serine proteases like trypsin and related enzymes.

Application

• Biochemical Research: As a fluorogenic substrate for kinetic assays and enzyme characterization, particularly for trypsin-like proteases.
• Pharmaceutical Screening: High-throughput screening (HTS) of potential protease inhibitors in drug discovery pipelines.
• Diagnostic Development: Used in the development of clinical assays to measure protease levels in biological samples.
• Enzyme Mechanism Studies: Investigating substrate specificity and catalytic efficiency of various proteolytic enzymes.
• Quality Control: Testing the activity and consistency of enzyme preparations in biomanufacturing.

Basic Information

Product Name	Ac-Phe-Arg-Amc Hcl
CAS No.	177028-04-5
Molecular Formula	C30H39N9O5 • xHCl
Molecular Weight	Contact for details
Synonyms	Acetyl-Phenylalanyl-Arginyl-7-Amino-4-Methylcoumarin Hydrochloride; N-Acetyl-L-phenylalanyl-L-arginine-7-amido-4-methylcoumarin hydrochloride; Ac-F-R-AMC • HCl; Trypsin Substrate V; Protease Fluorogenic Substrate; Boc-Phe-Ser-Arg-AMC (related); AMC-based protease substrate; Fluorogenic peptide substrate 177028-04-5.
EINECS	Contact for details

Quality Control

Our Ac-Phe-Arg-Amc Hcl is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.