Description

Biotin-PEG2-NH-Boc CAS NO 175885-18-4 is a high-purity, heterobifunctional crosslinker and bioconjugation reagent featuring a biotin moiety, a polyethylene glycol (PEG2) spacer, and a Boc-protected amine. This compound is engineered to facilitate the controlled and stable conjugation of biomolecules, enabling applications in targeted drug delivery, diagnostics, and proteomics research. It is an essential building block for researchers and manufacturers in the pharmaceutical, biotechnology, and life sciences sectors who require reliable, well-characterized reagents for advanced bioconjugation strategies.

Application

• ADC (Antibody-Drug Conjugate) Development: Used as a linker-payload intermediate for constructing stable, targeted cancer therapeutics.
• Diagnostic Assay Development: Serves as a key reagent for labeling proteins, antibodies, or peptides with biotin for use in ELISA, lateral flow assays, and other detection platforms.
• Affinity Purification Systems: Enables the immobilization of biomolecules onto streptavidin/avidin resins for pull-down assays and protein purification.
• Proteomics and Biomarker Research: Facilitates the tagging and isolation of specific proteins or cell surface receptors for mass spectrometry analysis.
• Nanoparticle Functionalization: Used to attach targeting ligands (via the amine) to drug-loaded nanoparticles, enhancing site-specific delivery.
• Chemical Biology Tool Development: Acts as a versatile scaffold for creating probes to study protein-protein interactions and cellular pathways.

Basic Information

Product Name	Biotin-PEG2-NH-Boc
CAS No.	175885-18-4
Molecular Formula	C23H43N5O6S
Molecular Weight	517.68 g/mol
Synonyms	Biotin-PEG2-amine-Boc; Boc-NH-PEG2-Biotin; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(2-((tert-butoxycarbonyl)amino)ethyl)pentanamide; 2-(2-((5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethoxy)ethyl)carbamic acid tert-butyl ester; Biotinamido-PEG2-amine-Boc; Biotin-PEG2-Boc-amine; Biotinylated PEG linker with Boc-protected amine
EINECS	Contact for details

Quality Control

Every batch of Biotin-PEG2-NH-Boc is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure identity, purity, and consistency. Our products are suitable for research and development use under cGMP guidelines where applicable. A detailed Certificate of Analysis (COA) providing results for assay, purity (HPLC), and identification is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Assay	≥ 97.0% (on anhydrous basis)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.