Description

β-D-Ribofuranoside, 4-nitrophenyl, 5-(dihydrogen phosphate) is a specialized nucleotide derivative and a key biochemical reagent. This compound matters as a critical substrate and intermediate in enzymatic assays and the synthesis of modified nucleotides. It is primarily needed by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for applications in enzymology, molecular biology, and drug discovery.

Application

• Enzymatic Substrate: Used as a chromogenic substrate for phosphatases, kinases, and other enzymes that act on phosphate esters, where the release of 4-nitrophenol is monitored spectrophotometrically.
• Nucleotide Synthesis: Serves as a vital phosphorylated building block in the chemical and enzymatic synthesis of nucleoside analogs, dinucleotides, and oligonucleotides.
• Biochemical Research: Employed in mechanistic studies of enzyme kinetics, inhibition, and signal transduction pathways involving phosphorylation events.
• Diagnostic Reagent: Utilized in the formulation of assay kits for clinical diagnostics and life science research to measure enzyme activity.
• Pharmaceutical Intermediate: Acts as a precursor in the development of prodrugs and antiviral or anticancer nucleoside therapeutics.

Basic Information

Product Name	β-D-Ribofuranoside, 4-nitrophenyl, 5-(dihydrogen phosphate)
CAS No.	174229-33-5
Molecular Formula	C11H14NO10P
Molecular Weight	351.21 g/mol
Synonyms	4-Nitrophenyl 5-O-Phosphono-β-D-ribofuranoside; p-Nitrophenyl 5-Phosphoribofuranoside; 4-Nitrophenyl β-D-Ribofuranoside 5-Phosphate; 5-O-Phosphono-β-D-ribofuranosyl 4-nitrobenzene; PNPRP; 4-Nitrophenyl β-D-Ribofuranoside 5-(Dihydrogen Phosphate); p-Nitrophenyl Ribofuranoside 5-Phosphate
EINECS	Contact for details

Quality Control

Our β-D-Ribofuranoside, 4-nitrophenyl, 5-(dihydrogen phosphate) is produced under a strict quality management system. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and mass spectrometry, to ensure it meets the high standards required for research and manufacturing. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic nature, the container should be kept in a desiccated environment to prevent moisture absorption. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Test in specified solvent

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.