Description

4-Nitrophenyl B-D-Xylotrioside is a specialized chromogenic substrate designed for the detection and quantification of specific glycosidase enzyme activity. This compound matters for researchers and quality control professionals who require precise, reliable, and sensitive biochemical assays. It is primarily needed by the life sciences, pharmaceutical, and diagnostic industries for applications in enzyme kinetics, inhibitor screening, and carbohydrate metabolism research.

Application

• Enzyme Assay Substrate: A critical reagent for the spectrophotometric measurement of β-xylosidase and related endo-xylanase activities.
• Biochemical Research: Used in studies of carbohydrate-active enzymes (CAZymes), including mechanism elucidation and kinetic parameter determination.
• Diagnostic Development: Serves as a key component in the development of diagnostic kits for enzyme deficiency disorders or microbial identification.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify potential glycosidase inhibitors for therapeutic development.
• Quality Control in Biotechnology: Used to standardize and validate enzyme preparations in industrial bioprocesses, such as those for biofuel or pulp/paper production.
• Microbiology Studies: Applied in research to characterize the glycosidase profiles of various microorganisms, including gut microbiota and plant pathogens.

Basic Information

Product Name	4-Nitrophenyl B-D-Xylotrioside
CAS No.	173468-29-6
Molecular Formula	C23H31NO16
Molecular Weight	577.49 g/mol
Synonyms	p-Nitrophenyl β-D-xylotrioside; 4-Nitrophenyl β-D-xylotrioside; PNPT; 4-NP-β-D-xylotrioside; pNP-Xylotrioside; 4-Nitrophenyl β-xylotrioside; 1,4-Bis(4-nitrophenoxy)-β-D-xylopyranosyl-(1→4)-β-D-xylopyranosyl-(1→4)-D-xylopyranose
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl B-D-Xylotrioside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture or light may affect stability and performance. For long-term storage, consider desiccant and under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO, DMF
Extinction Coefficient	~18,000 M-1cm-1 at 400-405 nm (in alkaline buffer)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.