Description

2,4-Dinitrophenyl β-D-Mannopyranoside is a specialized glycoside derivative used as a chromogenic substrate in enzymatic assays. This compound is critical for research and diagnostic applications where precise measurement of glycosidase enzyme activity is required. It is primarily utilized by biochemical researchers, diagnostic kit manufacturers, and pharmaceutical companies involved in enzyme inhibitor screening and carbohydrate metabolism studies.

Application

• Biochemical Research: As a chromogenic substrate for the detection and quantification of α-mannosidase and other glycosidase enzyme activities in vitro.
• Diagnostic Assays: A key component in enzymatic diagnostic kits for the study of lysosomal storage disorders, such as mannosidosis.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to identify and evaluate potential glycosidase inhibitors for therapeutic development.
• Enzyme Kinetics: Employed in studies to determine kinetic parameters (Km, Vmax) of mannosidases and related enzymes.
• Quality Control: Serves as a reference standard in the quality control of enzyme preparations and diagnostic reagents.
• Carbohydrate Chemistry Studies: A tool for investigating glycosidic bond cleavage mechanisms and substrate specificity of carbohydrate-active enzymes.

Basic Information

Product Name	2,4-Dinitrophenyl β-D-Mannopyranoside
CAS No.	171626-64-5
Molecular Formula	C12H14N2O10
Molecular Weight	346.25 g/mol
Synonyms	2,4-Dinitrophenyl β-D-Mannopyranoside; DNP-β-D-Mannopyranoside; p-Nitrophenyl β-D-Mannopyranoside (related); β-D-Mannopyranoside, 2,4-dinitrophenyl; Chromogenic Mannosidase Substrate; 2,4-DNP-β-Man; 171626-64-5; 4-Nitrophenyl β-D-mannopyranoside (structural analog)
EINECS	Contact for details

Quality Control

Our 2,4-Dinitrophenyl β-D-Mannopyranoside is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term storage, consider desiccant and under an inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	Yellow to light brown powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Melting Point	Contact for details
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.