Description

N4-Benzoyl-5'-O-(4,4'-Dimethoxytrityl)-2'-O-Propargyladenosine-3'-O-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite is a high-purity, protected phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound is specifically designed for the controlled introduction of modified adenosine residues, enabling the creation of advanced nucleic acid analogs for research and therapeutic applications. It is a critical raw material for pharmaceutical R&D, diagnostic developers, and biotechnology companies working on antisense oligonucleotides, siRNA, aptamers, and other next-generation genetic medicines.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key building block (amidite) in automated DNA/RNA synthesizers for the site-specific incorporation of propargyl-modified adenosine.
• Antisense & siRNA Therapeutics: Synthesis of research and therapeutic-grade oligonucleotides with tailored properties such as enhanced nuclease resistance or altered binding affinity.
• Diagnostic Probe Development: Manufacturing of labeled probes for fluorescence in situ hybridization (FISH), microarrays, and other molecular diagnostic assays.
• Aptamer Discovery: Construction of modified SELEX libraries to discover high-affinity aptamers with improved stability for therapeutic and sensing applications.
• Click Chemistry Conjugation: The 2'-O-propargyl group provides a handle for post-synthetic modification via copper-catalyzed azide-alkyne cycloaddition (CuAAC) to attach fluorophores, quenchers, or other functional groups.
• Academic & Industrial R&D: Fundamental research in nucleic acid chemistry, structure-activity relationship (SAR) studies, and the development of novel oligonucleotide-based technologies.

Basic Information

Product Name	N4-Benzoyl-5'-O-(4,4'-Dimethoxytrityl)-2'-O-Propargyladenosine-3'-O-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite
CAS No.	171486-59-2
Molecular Formula	C53H58N7O8P
Molecular Weight	952.05 g/mol
Synonyms	2'-O-Propargyl-5'-O-DMT-N6-Bz-adenosine-3'-CED phosphoramidite; 2'-O-Propargyl-N6-Benzoyl-5'-O-(4,4'-dimethoxytrityl)adenosine-3'-[(2-cyanoethyl)-N,N-diisopropyl]phosphoramidite; 2'-O-Propargyl-ABz Phosphoramidite; DMT-2'-O-Propargyl-N6-Bz-Adenosine-3'-CE Phosphoramidite; 2'-O-Propargyladenosine Phosphoramidite (N6-Bz, 5'-DMT); 5'-O-(4,4'-Dimethoxytrityl)-2'-O-propargyl-N6-benzoyladenosine-3'-O-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of our phosphoramidite monomers is manufactured and tested under strict quality control protocols to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Our production adheres to cGMP principles where applicable. Quality is verified by advanced analytical techniques including HPLC, 31P NMR, and 1H NMR. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content is provided with each shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation. For long-term stability, store in the original sealed packaging.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (31P NMR)	Spectrum corresponds to reference standard
Purity (HPLC)	≥ 97.0%
Moisture (Karl Fischer)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.