Description

25-Hydroxyvitamin D3 3-Glucuronide is a key conjugated metabolite of vitamin D3, formed via glucuronidation in the liver. This compound is of significant interest as a reference standard and biomarker for assessing vitamin D status and metabolism in clinical and research settings. It is primarily utilized by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic institutions focused on endocrinology and nutritional science.

Application

• Biomarker Reference Standard: Critical for the calibration and validation of analytical methods (LC-MS/MS, HPLC) used to quantify vitamin D metabolites in biological samples like serum and plasma.
• Clinical Diagnostics: Serves as a high-purity standard in the development and manufacturing of immunoassay and mass spectrometry-based diagnostic kits for accurate vitamin D status assessment.
• Pharmacokinetic & Metabolic Studies: Used in research to study the absorption, distribution, metabolism, and excretion (ADME) profiles of vitamin D3 and related analogs.
• Pharmaceutical Research: Employed as an intermediate or reference compound in the development of new therapeutic agents targeting vitamin D pathways.
• Nutritional Science Research: Facilitates studies on vitamin D bioavailability, conjugation pathways, and their implications for human health.
• Quality Control: Acts as a system suitability standard in laboratories to ensure the accuracy and precision of vitamin D metabolite analysis.

Basic Information

Product Name	25-Hydroxyvitamin D3 3-Glucuronide
CAS No.	170512-14-8
Molecular Formula	C33H52O9
Molecular Weight	592.76 g/mol
Synonyms	25-Hydroxyvitamin D3 3-β-D-Glucuronide; 25-Hydroxycholecalciferol 3-glucuronide; (3S,5Z,7E)-9,10-Secocholesta-5,7,10(19)-triene-3,25-diol 3-β-D-Glucopyranosiduronic Acid; 25(OH)D3-3G; Vitamin D3 metabolite 3-glucuronide; Calcidiol 3-glucuronide
EINECS	Contact for details

Quality Control

Our 25-Hydroxyvitamin D3 3-Glucuronide is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. A Certificate of Analysis (COA) detailing batch-specific results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in methanol, DMSO; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.