Description

5-Me-2'-Ome-U-Ce Phosphoramidite is a specialized, high-purity nucleoside phosphoramidite monomer used in automated oligonucleotide synthesis. This compound is critical for the solid-phase synthesis of modified RNA and DNA strands, enabling the precise incorporation of a 5-methyluridine derivative with a 2'-O-methyl ribose modification. It is an essential building block for research and development in therapeutic oligonucleotides, including antisense drugs, siRNA, and aptamers, where stability and specificity are paramount. Leading users include pharmaceutical R&D teams, biotechnology firms, and CROs specializing in nucleic acid chemistry.

Application

• Therapeutic Oligonucleotide Synthesis: Key monomer for synthesizing siRNA, antisense oligonucleotides (ASOs), and aptamers with enhanced nuclease resistance and binding affinity.
• Research Probe Development: Used to create labeled or modified probes for genomics, transcriptomics, and molecular diagnostics.
• Structural Biology Studies: Incorporation into RNA strands to study structure-function relationships and RNA-protein interactions.
• Pre-clinical Drug Development: Essential raw material for the GMP-compliant production of oligonucleotide candidates for in vitro and in vivo testing.
• Diagnostic Assay Components: Manufacturing of specialized oligonucleotides used in PCR primers, molecular beacons, and other diagnostic tools.

Basic Information

Product Name	5-Me-2'-Ome-U-Ce Phosphoramidite
CAS No.	168611-18-5
Molecular Formula	C33H41N4O8P
Molecular Weight	652.68 g/mol
Synonyms	5-Methyl-2'-O-methyluridine-CE Phosphoramidite; 2'-O-Methyl-5-methyluridine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); 5-Me-2'-OMe-U-CE Phosphoramidite; 2'-O-Methyl-5-methyluridine CE Phosphoramidite; 5-Methyl-2'-O-methyl-Uridine Phosphoramidite; 2'-O-Me-5-Me-U Phosphoramidite; Uridine, 2'-O-methyl-5-methyl-, 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite)
EINECS	Contact for details

Quality Control

Every batch of our 5-Me-2'-Ome-U-Ce Phosphoramidite is manufactured and tested under strict quality systems to ensure consistency and reliability for critical oligonucleotide synthesis. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing purity, identity, and moisture content. Our quality protocols are designed to meet the stringent requirements of pharmaceutical R&D and GMP-grade production. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will lead to decomposition and reduced coupling efficiency. Allow sealed vials to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Moisture Content (KF)	≤ 1.0%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.