Description

Benzyloxycarbonyl-L-Leucyl-L-Arginylglycylglycine 4-Methylcoumaryl-7-Amide is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise detection and quantification of specific protease activity. It is primarily utilized in biochemical research, pharmaceutical development, and diagnostic kit manufacturing for studying enzyme kinetics and inhibitor screening.

Application

• Protease Activity Assays: As a highly specific fluorogenic substrate for measuring the activity of enzymes like trypsin, thrombin, and other serine proteases.
• Biochemical Research: Used in kinetic studies, enzyme characterization, and mechanistic investigations within academic and industrial R&D settings.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and evaluate potential protease inhibitors as drug candidates.
• Diagnostic Development: Integral component in the development of clinical diagnostic assays and kits for detecting protease-related biomarkers.
• Quality Control: Serves as a reference standard in the QC of biopharmaceuticals, particularly those involving recombinant proteases.

Basic Information

Product Name	Benzyloxycarbonyl-L-Leucyl-L-Arginylglycylglycine 4-Methylcoumaryl-7-Amide
CAS No.	167698-68-2
Molecular Formula	C₃₃H₄₈N₁₀O₈
Molecular Weight	712.81 g/mol
Synonyms	Z-Leu-Arg-Gly-Gly-AMC; Z-Leu-Arg-Gly-Gly-7-amido-4-methylcoumarin; Cbz-Leu-Arg-Gly-Gly-AMC; Fluorogenic Substrate for Trypsin; Boc-Leu-Arg-Gly-Gly-AMC (common variant); L-Leucine, N-[(phenylmethoxy)carbonyl]-L-leucyl-N5-(N,N'-bis[(aminoiminomethyl)amino]-L-ornithyl)glycylglycyl-, 7-amido-4-methylcoumarin amide; AMC-labeled peptide substrate; Protease Substrate IV
EINECS	Contact for details

Quality Control

Our Benzyloxycarbonyl-L-Leucyl-L-Arginylglycylglycine 4-Methylcoumaryl-7-Amide is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference standard in fluorogenic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.