Description

Uridine, 5'-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-5-Bromo-2'-o-[(1,1-Dimethylethyl)Dimethylsilyl]-, 3'-[2-Cyanoethyl Bis(1-Methylethyl)Phosphoramidite] (9Ci) is a highly specialized, protected nucleoside phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. This compound is engineered for precise and efficient incorporation of modified uridine bases into synthetic DNA or RNA strands, enabling advanced research and therapeutic development. It is essential for pharmaceutical R&D, biotechnology, and academic laboratories focused on antisense oligonucleotides, siRNA, aptamers, and diagnostic probes.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of modified DNA/RNA sequences.
• Therapeutic Development: Production of antisense oligonucleotides and siRNA candidates for targeted gene silencing therapies.
• Research Reagent: Used in academic and industrial R&D to study nucleic acid structure, function, and protein interactions.
• Diagnostic Probe Manufacturing: Creation of labeled probes for genomics, molecular diagnostics, and fluorescence in situ hybridization (FISH).
• Aptamer Synthesis: Building block for generating nucleic acid aptamers used in biosensing and targeted drug delivery.
• CRISPR/Cas9 System Enhancement: Synthesis of modified guide RNAs (gRNAs) to improve stability and specificity.

Basic Information

Product Name	Uridine, 5'-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-5-Bromo-2'-o-[(1,1-Dimethylethyl)Dimethylsilyl]-, 3'-[2-Cyanoethyl Bis(1-Methylethyl)Phosphoramidite] (9Ci)
CAS No.	166733-98-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5'-DMT-5-Bromo-2'-TBDMS-uridine-3'-CE phosphoramidite; 5-BrU-CE Phosphoramidite; 5-Bromo-2'-deoxyuridine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite), 5'-DMT protected; 5-Bromo-2'-O-(tert-butyldimethylsilyl)-5'-O-(4,4'-dimethoxytrityl)uridine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); 5-Br-dU Phosphoramidite; 5-Bromo-uridine phosphoramidite; Amidite, 5-Br-dU
EINECS	Contact for details

Quality Control

Our high-purity phosphoramidite monomers are produced under strict quality-controlled environments to ensure batch-to-batch consistency and optimal coupling efficiency. Each lot undergoes comprehensive analysis, including HPLC for purity and 31P NMR for identity and stability. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming specifications for critical parameters such as assay, moisture content, and purity. We support compliance with GMP and ISO 9001 standards for research and development use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms
Identification (31P NMR)	Conforms
Purity (HPLC)	≥ 97.0%
Assay	≥ 95.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.