Description

2-[2-[2-(2-T-Boc-Aminoethoxy]Ethoxy]Ethoxy]-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoic Acid, Methyl Ester is a sophisticated bifunctional chemical building block that integrates a photolabile diazirine group with a cleavable T-Boc protected amine linker via a tetraethylene glycol spacer. This compound matters for its unique ability to serve as a photoactivatable crosslinking agent in advanced biochemical research, enabling the study of protein-protein interactions and molecular labeling. It is primarily needed by researchers and manufacturers in pharmaceutical R&D, proteomics, and chemical biology for developing targeted therapies and diagnostic tools.

Application

• Photoaffinity Labeling (PAL): A critical reagent for covalent capture and identification of transient or weak biological interactions in drug target discovery.
• Chemical Proteomics: Used to profile enzyme activities and map small molecule-protein interactions within complex cellular systems.
• Bioconjugation & Linker Chemistry: Serves as a versatile heterobifunctional crosslinker for attaching probes, drugs, or affinity tags to biomolecules.
• Prodrug Synthesis: Employed in the design of light-activated prodrugs and therapeutic agents for controlled release applications.
• Materials Surface Functionalization: Used to create bioactive surfaces on nanoparticles, chips, or polymers for biosensing and diagnostic devices.
• Fragment-Based Drug Discovery (FBDD): Acts as a photoreactive fragment for covalent library screening against protein targets.

Basic Information

Product Name	2-[2-[2-(2-T-Boc-Aminoethoxy]Ethoxy]Ethoxy]-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoic Acid, Methyl Ester
CAS No.	165963-73-5
Molecular Formula	C24H32F3N3O7
Molecular Weight	531.53 g/mol
Synonyms	Methyl 4-[3-(trifluoromethyl)diazirin-3-yl]-2-[2-[2-[2-[(2-methylpropan-2-yl)oxycarbonylamino]ethoxy]ethoxy]ethoxy]benzoate; T-Boc-Amino-PEG4-Diazirine-Methyl Ester; Photoactivatable T-Boc-PEG4 Crosslinker; 3-(Trifluoromethyl)-3H-diazirin-3-yl-PEG4-T-Boc Methyl Ester; BocNH-PEG4-Diazirine Methyl Ester; 4-(3-(Trifluoromethyl)-3H-diazirin-3-yl)benzoic Acid, Methyl Ester, PEG4-T-Boc Amine Derivative; 165963-73-5
EINECS	Contact for details

Quality Control

Our production of this high-value biochemical reagent adheres to strict quality protocols to ensure batch-to-batch consistency and research reproducibility. Quality is verified through comprehensive analytical techniques including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, and suitability for sensitive biochemical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a desiccated environment. This compound is strictly light-sensitive and hygroscopic; prolonged exposure to light, moisture, or ambient conditions will lead to decomposition. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMSO, DMF, acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.