Description

Phosphoramidous Acid, Bis(1-Methylethyl)-, 22,22-Bis(4-Methoxyphenyl)-22-Phenyl-3,6,9,12,15,18,21-Heptaoxadocos-1-Yl 2-Cyanoethyl Ester is a specialized phosphoramidite monomer, a critical building block in the automated synthesis of oligonucleotides. Its complex structure, featuring a long polyethylene glycol (PEG) linker and photolabile protecting groups, is engineered for advanced applications requiring solubility and controlled reactivity. This high-purity intermediate is essential for researchers and manufacturers in the fields of therapeutic oligonucleotide development, diagnostic probe synthesis, and advanced genetic research.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for introducing modified nucleosides into DNA/RNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides, siRNA, and aptamers for pharmaceutical applications.
• Diagnostic Probe Development: Synthesis of labeled probes for fluorescence in situ hybridization (FISH) and microarray technologies.
• Research-Grade Reagent: For academic and industrial R&D focused on nucleic acid chemistry and novel biomaterials.
• Conjugation Chemistry: The extended PEG chain facilitates the synthesis of oligonucleotide-polymer conjugates to enhance pharmacokinetic properties.
• Photolabile Protecting Group Strategy: Enables light-directed deprotection in spatially controlled synthesis applications.

Basic Information

Product Name	Phosphoramidous Acid, Bis(1-Methylethyl)-, 22,22-Bis(4-Methoxyphenyl)-22-Phenyl-3,6,9,12,15,18,21-Heptaoxadocos-1-Yl 2-Cyanoethyl Ester
CAS No.	165682-45-1
Molecular Formula	C55H76N2O12P
Molecular Weight	997.18 g/mol
Synonyms	5'-(4,4'-Dimethoxytrityl)-2'-deoxy-5-methyluridine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite, PEGylated version; DMT-dT(CEDP) Phosphoramidite, PEGylated; Photolabile PEG Phosphoramidite; 5'-DMT-Thymidine 3'-CE Phosphoramidite with Photocleavable Linker; Oligonucleotide Synthesis Monomer, Photocleavable; 2-Cyanoethyl N,N-diisopropylphosphoramidite derivative of protected thymidine PEG conjugate.
EINECS	Contact for details

Quality Control

Every batch of this high-value phosphoramidite is produced and tested under strict inert atmosphere conditions to prevent oxidation and hydrolysis. Quality is assured through comprehensive analytical methods including HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and stability. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting critical parameters such as assay purity, moisture content (Karl Fischer), and solvent residues. Our quality system is designed to meet the exacting standards required for GMP-grade oligonucleotide synthesis.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized (store under inert atmosphere). Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation. For long-term stability, store in the original sealed packaging.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 0.5%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.