Description

2-Chloro-4-Nitrophenyl-β-D-Maltotrioside is a specialized chromogenic substrate designed for the sensitive detection and quantification of enzymatic activity. This compound matters because it provides a reliable and measurable colorimetric signal upon enzymatic cleavage, enabling precise biochemical analysis. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries for enzyme assay development and screening applications.

Application

• Substrate for α-Glucosidase Enzymes: Primary use as a chromogenic substrate for the assay of α-glucosidase activity, including sucrase-isomaltase and other related enzymes.
• Biochemical Research & Development: Essential tool in enzymology studies for kinetic analysis, inhibitor screening, and characterizing enzyme mechanisms.
• Diagnostic Reagent Manufacturing: Used in the formulation of diagnostic kits for the detection of enzyme deficiencies or related metabolic disorders.
• Pharmaceutical Quality Control: Employed in potency testing and stability studies of enzyme-based pharmaceuticals.
• High-Throughput Screening (HTS): Suitable for automated screening platforms in drug discovery to identify modulators of glycosidase activity.
• Food & Beverage Analysis: Potential application in assessing enzyme activity in industrial processes like starch conversion.

Basic Information

Product Name	2-Chloro-4-Nitrophenyl-β-D-Maltotrioside
CAS No.	165522-16-7
Molecular Formula	C30H44ClNO23
Molecular Weight	806.11 g/mol
Synonyms	4-Nitrophenyl 2-chloro-4-nitrophenyl β-D-maltotrioside; CNP-G3; 2-Chloro-4-nitrophenyl β-maltotrioside; 2-Chloro-4-nitrophenyl maltotrioside; α-Glucosidase substrate CNP-G3; Chromogenic α-glucosidase substrate; p-Nitrophenyl 2-chloro-4-nitrophenyl β-D-maltotrioside
EINECS	Contact for details

Quality Control

Our 2-Chloro-4-Nitrophenyl-β-D-Maltotrioside is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.