Description

Nod Factor V, Bradyrhizobium Japonicum CAS NO 163181-91-7 is a purified lipochitooligosaccharide signaling molecule, specifically a Nod factor, produced by the nitrogen-fixing bacterium *Bradyrhizobium japonicum*. This compound plays a critical role in initiating the symbiotic relationship between the bacterium and legume roots, such as soybeans, leading to the formation of nitrogen-fixing nodules. It is an essential research tool for plant biologists, microbiologists, and agricultural scientists studying plant-microbe interactions, symbiosis signaling pathways, and the development of next-generation biofertilizers. The high-purity grade ensures reliable and reproducible results in both fundamental research and applied agricultural biotechnology.

Application

• Symbiosis Research: Fundamental studies on the molecular dialogue and signaling mechanisms between rhizobia and legume hosts.
• Plant Physiology Studies: Investigation of root hair deformation, cortical cell division, and early nodulation events in model and crop legumes.
• Biofertilizer Development: Used as a reference standard and active component in research aimed at enhancing the efficiency of microbial inoculants and biostimulants.
• Receptor-Ligand Interaction Analysis: Essential for characterizing plant LysM receptor kinases and understanding specificity in symbiotic recognition.
• Agricultural Biotechnology: Applied research to improve nitrogen fixation efficiency in soybean and other legume crops under field conditions.
• Biochemical Assays: Serves as a critical reagent in enzymatic assays, binding studies, and structural biology research related to nodulation factors.

Basic Information

Product Name	Nod Factor V, Bradyrhizobium Japonicum
CAS No.	163181-91-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Nod Bj-V (Nodulation Factor); Lipo-chitooligosaccharide (LCO) from B. japonicum; Bradyrhizobium japonicum Nod Factor V; Nod Bj V; Nod factor (B. japonicum); Symbiotic signaling molecule; Nodulation signal; LCO-V
EINECS	Contact for details

Quality Control

Our Nod Factor V is produced and handled under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous analytical characterization, including purity assessment via HPLC and structural confirmation via mass spectrometry (MS) and/or NMR. Certificates of Analysis (COA) detailing purity, identity, and specific activity are available upon request to support your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, keep desiccated at -80°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Biological Activity (Root Hair Assay)	Active at pM-nM concentrations
Solubility	Soluble in water, DMSO, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.