Description

2-Chloro-4-Nitrophenyl β-D-Cellotrioside is a specialized chromogenic substrate designed for the sensitive and specific detection of cellulase enzyme activity. This compound matters because it provides a reliable, colorimetric readout for quantifying endo-1,4-β-glucanase activity, a critical parameter in enzymatic research and industrial biocatalysis. It is primarily needed by researchers in enzymology, biotechnology firms developing enzyme cocktails for biomass conversion, and quality control laboratories in the biofuel, textile, and pulp & paper industries.

Application

• Enzymatic Assay Substrate: Primary use as a chromogenic substrate for the specific assay of endo-1,4-β-glucanase (cellulase) activity in research and industrial samples.
• Biomass Conversion Research: Used to screen and characterize novel cellulolytic enzymes or engineered enzyme cocktails for lignocellulosic biomass degradation in biofuel production.
• Quality Control in Enzyme Production: Employed by manufacturers to standardize and verify the activity of commercial cellulase preparations.
• Microbiological Studies: Applied in research to study cellulase production by microorganisms, including fungi and bacteria.
• Textile and Pulp & Paper Industry R&D: Utilized in developing and optimizing enzymatic processes for biostoning of denim or bio-pulping.
• Diagnostic Reagent Development: Serves as a key component in developing diagnostic kits for detecting specific enzymatic activities in clinical or environmental samples.

Basic Information

Product Name	2-Chloro-4-Nitrophenyl β-D-Cellotrioside
CAS No.	161597-23-5
Molecular Formula	C30H44ClNO23
Molecular Weight	806.12 g/mol
Synonyms	4-Nitrophenyl 2-chloro-4-nitrophenyl β-D-cellotrioside; 2-Chloro-4-nitrophenyl β-cellotrioside; CNP-Cellotrioside; β-4,2'-Chloronitrophenyl cellotrioside; 2-Chloro-4-nitrophenyl β-D-glucopyranosyl-(1→4)-β-D-glucopyranosyl-(1→4)-β-D-glucopyranoside; Chromogenic cellulase substrate; Cellulase substrate CNP-C3
EINECS	Contact for details

Quality Control

Our 2-Chloro-4-Nitrophenyl β-D-Cellotrioside is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive enzymatic assays. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and performance consistency for your research and development needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light may lead to decomposition and reduced performance. For long-term stability, consider storage under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO
Biological Activity	Suitable for cellulase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.