Description

2,5-Dioxopyrrolidin-1-Yl 4-[(Pyridin-2-Yldisulfanyl)Methyl]Benzoate is a specialized heterobifunctional crosslinking reagent featuring both an amine-reactive N-hydroxysuccinimide (NHS) ester and a sulfhydryl-reactive pyridyl disulfide group. This compound is essential for creating stable, cleavable conjugates between proteins, peptides, or other amine-containing molecules and thiols, enabling advanced bioconjugation strategies. It is a critical tool for researchers and manufacturers in biopharmaceutical development, proteomics, and diagnostic assay production, where precise and reversible linkage of biomolecules is required.

Application

• Bioconjugation and Protein Modification: For site-specific crosslinking of antibodies, enzymes, or other proteins to carriers, surfaces, or other biomolecules via amine-to-thiol coupling.
• Drug-Linker Conjugation in ADC Development: As a key chemical linker component in the synthesis of Antibody-Drug Conjugates (ADCs), connecting cytotoxic payloads to monoclonal antibodies.
• Immobilization Chemistry: Functionalizing gold surfaces, nanoparticles, or chromatographic supports with biomolecules for biosensor and diagnostic device fabrication.
• Peptide Synthesis and Labeling: Introducing cleavable disulfide linkages or fluorescent/affinity tags onto synthetic peptides.
• Polymer and Biomaterial Science: Crosslinking hydrogels or functional polymers to create smart materials with responsive or degradable properties.
• Proteomics Research: For capturing, isolating, and studying thiol-containing proteins or peptides in complex biological samples.

Basic Information

Product Name	2,5-Dioxopyrrolidin-1-Yl 4-[(Pyridin-2-Yldisulfanyl)Methyl]Benzoate
CAS No.	160854-54-6
Molecular Formula	C17H14N2O4S2
Molecular Weight	374.43 g/mol
Synonyms	4-[(Pyridin-2-Yldisulfanyl)Methyl]Benzoic Acid N-Hydroxysuccinimide Ester; SPDP-NHS; N-Succinimidyl 3-(2-pyridyldithio)propionate Benzoate Analog; NHS-Pyridyldithiopropionate; N-Succinimidyl 4-((pyridin-2-yldisulfanyl)methyl)benzoate; Heterobifunctional Crosslinker SPDP-NHS; 4-((2-Pyridinyldithio)methyl)benzoic Acid N-Hydroxysuccinimide Ester; 4-(Pyridin-2-yldisulfanylmethyl)benzoic Acid 2,5-Dioxopyrrolidin-1-yl Ester
EINECS	Contact for details

Quality Control

Our production of 2,5-Dioxopyrrolidin-1-Yl 4-[(Pyridin-2-Yldisulfanyl)Methyl]Benzoate adheres to stringent quality management protocols. Each batch is characterized and tested to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) detailing identity, purity (HPLC), and functional group integrity are provided with every shipment. We support compliance with cGMP and ISO 9001 standards for research and pharmaceutical-grade materials.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant (e.g., in a sealed bag with silica gel) due to its hygroscopic and moisture-sensitive nature. For long-term storage, keep under inert atmosphere (argon or nitrogen) to prevent oxidation of the disulfide bond. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.