Description

5-Bromo-4-Chloro-3-Indolyl-α-D-n-Acetylneuraminic Acid Sodium Salt is a specialized chromogenic substrate used for the detection of neuraminidase (sialidase) activity. This compound matters for its high sensitivity and specificity in enzymatic assays, enabling precise research and diagnostic applications. It is primarily needed by biochemical researchers, diagnostic kit manufacturers, and pharmaceutical developers working in virology, bacteriology, and glycobiology.

Application

• Enzymatic Substrate for Neuraminidase/Sialidase Detection: Used as a key reagent in colorimetric and fluorometric assays to detect and quantify neuraminidase activity from viral, bacterial, or mammalian sources.
• Virology and Antiviral Research: Essential for studying influenza virus neuraminidase function and for screening potential antiviral compounds that inhibit this enzyme.
• Diagnostic Kit Manufacturing: Incorporated into commercial diagnostic kits for the detection of pathogens like influenza viruses or certain bacteria that produce sialidase.
• Glycobiology and Cell Biology Studies: Employed to investigate the role of sialic acids and their cleavage in cell signaling, adhesion, and immune response.
• Microbiology Research: Used to characterize sialidase-producing bacteria, such as Clostridium perfringens and other pathogens.
• Quality Control in Biologics: Applied in the QC testing of vaccines and other biologics to ensure the absence of contaminating neuraminidase activity.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl-α-D-n-Acetylneuraminic Acid Sodium Salt
CAS No.	160369-85-7
Molecular Formula	C20H24BrClN2NaO10
Molecular Weight	590.76 g/mol
Synonyms	X-Neu5Ac Sodium Salt; 5-Bromo-4-chloro-3-indolyl-α-D-N-acetylneuraminate Sodium Salt; 5-Bromo-4-chloro-1H-indol-3-yl α-D-N-acetylneuraminic acid Sodium Salt; 5-Bromo-4-chloro-3-indolyl-α-D-N-acetylneuraminic acid Sodium Salt; BCI-Neu5Ac Na; Chromogenic Neuraminidase Substrate; Indoxyl-Neu5Ac Sodium Salt
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl-α-D-n-Acetylneuraminic Acid Sodium Salt is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Suitable for neuraminidase detection

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.