Description

trans-3'-Hydroxycotinine, Methyl-D3 is a stable isotope-labeled internal standard of a major nicotine metabolite. This compound is essential for ensuring the accuracy and reliability of quantitative bioanalytical assays. It is primarily required by analytical laboratories and research institutions engaged in pharmacokinetic studies, forensic toxicology, and clinical research related to nicotine metabolism and exposure.

Application

• Primary use as a stable isotope-labeled internal standard for the precise quantification of 3'-hydroxycotinine in biological matrices via LC-MS/MS.
• Critical component in pharmacokinetic and metabolic profiling studies of nicotine and its derivatives.
• Enhances method validation for forensic and clinical toxicology testing, improving result defensibility.
• Supports biomarker research investigating smoking behavior, cessation efficacy, and associated health risks.
• Used in the development and calibration of diagnostic assays and reference materials.
• Facilitates regulatory compliance testing in workplace nicotine exposure monitoring and related public health studies.

Basic Information

Product Name	trans-3'-Hydroxycotinine, Methyl-D3
CAS No.	159956-78-2
Molecular Formula	C10H10D3NO2
Molecular Weight	180.23 g/mol
Synonyms	(3'R,5'S)-3'-Hydroxycotinine-methyl-d3; 5-(3-Hydroxy-1-methyl-2-pyrrolidinyl)-2-pyridinol-methyl-d3; 3'-Hydroxycotinine (methyl-d3); trans-3'-Hydroxycotinine-d3 (methyl labeled); Nicotine metabolite-d3; Cotinine metabolite-d3; 3HC-d3; HYC-d3
EINECS	Contact for details

Quality Control

Our trans-3'-Hydroxycotinine, Methyl-D3 is manufactured under strict quality systems to ensure high chemical and isotopic purity, critical for its role as an analytical standard. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing results for identity, purity (by HPLC), isotopic enrichment, and related substances. We adhere to relevant guidelines for the production of reference standards to support GLP/GMP-compliant research and testing.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Chemical Purity (by HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.